Determination of Lead Phytoextraction Capacity of Transgenic (p-cV-ChMTIIGFP) Tobacco Plant and Effect of Lead on Nutrient Uptake
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https://doi.org/10.5281/zenodo.13329516Keywords:
Heavy metal, phytoremediation, lead (Pb), soil pollution, transgenic, tobaccoAbstract
This study aimed to assess the lead phytoextraction capacity of transgenic (p-cV-ChMTIIGFP) tobacco plants grown in soil contaminated with lead (Pb), as well as to examine how Pb affects the uptake of macro- and micronutrients by these plants, in comparison with non-transgenic SR-1 tobacco plants. The soil was administered increasing doses of Pb in the form of Pb(NO3)2, and the plants were cultivated under controlled conditions for a period of six weeks. The impact of the lead treatments on the Pb content of the plants was found to be statistically significant at the 5% level. The highest Pb content (53.2 µg plant-1) was observed in the 400 mg kg-1 application of the transgenic plant. The results indicated that increasing doses of Pb led to reductions in the concentrations of N, P and K in both plant types in comparison with the control. The application of Pb at increasingly elevated doses to the soil led to a statistically significant elevation in the concentrations of both Cu and Zn in transgenic and non-transgenic tobacco plants, as compared to the concentrations in the control plants. The application of increasing doses of Pb to soil resulted in a statistically significant increase in the concentrations of Cu and Zn in transgenic and non-transgenic tobacco plants, when compared to the concentrations in the control plants. The effect of Pb applications on the concentrations of iron (Fe) and manganese (Mn) was found to be statistically insignificant. It was found that transgenic (p-cV-ChMTIIGFP) tobacco plants did not show toxic symptoms despite Pb stress and the reduction effect on nutrient uptake has the potential to increase the accumulation capacity by supporting fertilization.
References
ATSDR, 2024. The ATSDR 2022 Substance Priority List. (https://www.atsdr.cdc.gov/spl/index.html#2022spl), Erişim: 07.03.2024.
Baker, A.J.M., Reeves, R.D., McGrath, S.P., 1991. In situ decontamination of heavy metal polluted soils using crops of metal-accumulating plants - A feasibility study. In: Hinchee, R.E., Olfenbuttel, R.F. (Eds.), In situ bioreclamation: applications and investigations for hydrocarbon and contaminated sites remediation. Butterworth-Heinemann, London, pp. 600-605.
Bouyoucos, G.J., 1951. A recalibration of hydrometer for making mechanical analysis of soils. Agronomy Journal, 43: 434-438.
Burton, G.W., Ingold, K.U., 1984. Carotene: An unusual type of lipid antioxidant. Science, 224: 569-573.
Chatterjee, C., Dube, B.K., Sinha, P., Srivastava, P., 2004. Detrimental effects of lead phytotoxicity on growth, yield, and metabolism of rice. Communications in Soil Science and Plant Analysis, 35(1-2): 255-265.
Dağhan, H., 2004. Phytoextraction of heavy metal from contaminated soils using genetically modified plants. PhD thesis, RWTH-Aachen University, Germany.
Dağhan, H., 2007. Fitoremediasyon: Bitki kullanılarak kirlenmiş toprakların temizlenmesi. GAP Tarım Kongresi-V, Kongre Bildiri Kitabı, 17-19 Ekim 2007), Şanlıurfa, s. 362-367.
Dalyan, E., Yüzbaşıoğlu, E., Akpınar, I., 2020. Physiological and biochemical changes in plant growth and different plant enzymes in response to lead stress. In: B. Fagan, ed. Lead in Plants and the Environment. London.
Doğan, M., Colak, U., 2009. Effect of lead applied to Triticum aestivum L. cv. Tosunbey on some physiological characteristics. Ekoloji, 19(73): 98-104.
Dotaniya, M.L., Dotaniya, C.K., Solanki, P., Meena, V.D., Doutaniya, R.K., 2020. Lead contamination and its dynamics in soil–plant system. In: B. Fagan, ed. Lead in Plants and the Environment. London, pp, 83-98.
Egendorf, S.P., Groffman, P., Moore, G., Cheng, Z., 2020. The limits of lead (Pb) phytoextraction and possibilities of phytostabilization in contaminated soil: a critical review. International Journal of Phytoremediation, 22(9): 916–930.
EPA, 1995. Contaminants and remedial options at select metals-Contaminated Sites, EPA/540/R-95/512.6.
Eren, A., 2010. Kurşun metali ile kirlenmiş toprakların transgenik bitki kullanılarak temizlenme olanaklarının araştırılması. Yüksek Lisans Tezi, Mustafa Kemal Üniversitesi, Fen Bilimleri Enstitüsü. Hatay.
Eren, A., Mert, M., 2017. Ağır metal (Ni, Cd ve Cu) uygulamalarının Andız Otu, Fener Otu ve Sığırkuyruğu bitkilerinin büyüme ve gelişmesi üzerine etkisi. Türkiye Tarımsal Araştırmalar Dergisi, 4(1): 50-58.
Gisbert, C., Ros, R., De Haro, A., Walker, D.J., Bernal, M.P., Serrano, R., Navarro-Aviñó, J., 2003. A plant genetically modified that accumulates Pb is especially promising for phytoremediation. Biochem. Biophys. Res. Commun., 303(2): 440-445.
Gopal, R., Rizvi, A.H., 2008. Excess lead alters growth, metabolism and translocation of certain nutrients in radish. Chemosphere, 70(9): 1539-1544.
Jagetiya, B., Kumar, S., 2020. Phytoremediation of lead: a review. In: B. Fagan, ed. Lead in Plants and the Environment. London.
Jones, J.B. Jr., Wolf, B., Mills, H.A., 1991. Plant Analysis Handbook: A Practical Sampling, Preparation, Analysis and Interpretation Guide. Athens, GA: Micro - Macro Publishing.
Jones, L.H.P., Clement, C.R., Hopper, M.J., 1973. Lead uptake from solution by perennial ryegrass and its transport from roots to shoots. Plant Soil, 38: 403-414.
Kabata-Pendias, A., 1992. Trace metals in soils in Poland—occurrence and behaviour. Soil Science Journal, 140: 53-70.
Kabata-Pendias, A., Pendias, H., 2001. Trace elements in Soils and Plants. 3rd Edn. Boca Raton: CRC Press. London.
Kacar, B., 1994. Bitki ve Toprağın Kimyasal Analizleri. Ankara: Ankara Üniversitesi Ziraat Fakültesi Eğitim, Araştırma ve Geliştirme Vakfı. No: 3, Ankara, s,705.
Kınay, A., Erdem, H., 2019. The Effects of Increasing Doses of Lead Applications on Growth, Pb And Microelement Concentrations of Tobacco Varieties. Turkish Journal of Agriculture - Food Science and Technology, 7(12): 2083-2088.
Lindsay, W.L., 1979. Chemical Equilibria in Soils. N.Y.: Wiley and Sons. p, 449.
Lindsay, W.L., Norvell, W.A., 1978. Development of a DTPA test for zinc, iron, manganese, and copper. Soil Science Society of America Journal, 42: 421-428.
Loeppert, R.H., Suarez, D.L., 1996. Carbonate and gypsum. In: D.L. Spark (Ed.), Methods of soil analysis. Part 3. Chemical Methods. 5: 437-474.
Masindi, V., Mkhonza, P., Tekere, M., 2021. Sources of heavy metals pollution. In: B. Fagan, ed. Remediation of Heavy Metals. London.
Meena, V., Dotaniya, M.L., Saha, J.K., Das, H., Patra, A.K., 2020. Impact of lead contamination on agroecosystem and human health. In: B. Fagan, ed. Lead in Plants and the Environment, 67-82.
Rahman, S.U., Qin, A., Zain, M., Mushtaq, Z., Mehmood, F., Riaz, L., Naveed, S., Ansari, M.J., Saeed, M., Ahmad, I., Shehzad, M., 2024. Lead uptake, accumulation, and translocation in plants: Plant physiological, biochemical, and molecular response: A review. Ecotoxicology and Environmental Safety, 241: 110849.
Raychaudhuri, S.S., Pramanick, P., Talukder, P., Basak, A., 2021. Polyamines, metallothioneins, and phytochelatins—Natural defense of plants to mitigate heavy metals. Studies in Natural Products Chemistry, 69: 227-261.
Richards, L.A., 1954. Diagnosis and Improvement of Saline and Alkali Soils. United States Department of Agriculture Handbook 60: 94.
Rigoletto, M., Calza, P., Gaggero, E., Malandrino, M., Fabbri, D., 2020. Bioremediation methods for the recovery of lead-contaminated soils: A review. Applied Sciences, 10(10): 3528.
Ruttens, A., Boulet, J., Weyens, N., Smeets, K., Adriaensen, K., Meers, E., Vangronsveld, J., 2011. Short rotation coppice culture of willows and poplars as energy crops on metal contaminated agricultural soils. International Journal of Phytoremediation, 13(1): 194-207.
Sharma, P., Dubey, R. S., 2005. Lead toxicity in plants. Brazilian Journal of Plant Physiology, 17(1): 35-52.
Song, W.Y., Sohn, E.J., Martinoia, E., Lee, Y.J., Yang, Y.Y., Jasinski, M., Forestier, C., Hwang, I., Lee, Y., 2003. Engineering tolerance and accumulation of lead and cadmium in transgenic plants. Nature Biotechnology, 21(8): 914-919.
Taciroglu, B., Kara, E., Sak, T., 2016. Toprakta Ağır Metal Gideriminde Solucanların Kullanımı. Kahramanmaraş Sütcü İmam Üniversitesi Doğa Bilimleri Dergisi, 19(2): 201-207.
Wallace, A., Wallace, G.A., Cha, J.W., 1992. Some modifications in trace metal toxicities and deficiencies in plants resulting from interactions with other elements and chelating agents‐‐the special case of iron. Journal of Plant Nutrition, 15(10): 1589-1598.
Yilmaz, K., Akinci, İ. E., Akinci, S., 2009. Effect of lead accumulation on growth and mineral composition of eggplant seedlings (Solanum melongena). New Zealand Journal of Crop and Horticultural Science, 37(3): 189-199.
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