Determination of Lead Phytoextraction Capacity of Transgenic (p-cV-ChMTIIGFP) Tobacco Plant and Effect of Lead on Nutrient Uptake
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https://doi.org/10.5281/zenodo.13329516Keywords:
Heavy metal, phytoremediation, lead (Pb), soil pollution, transgenic, tobaccoAbstract
This study aimed to assess the lead phytoextraction capacity of transgenic (p-cV-ChMTIIGFP) tobacco plants grown in soil contaminated with lead (Pb), as well as to examine how Pb affects the uptake of macro- and micronutrients by these plants, in comparison with non-transgenic SR-1 tobacco plants. The soil was administered increasing doses of Pb in the form of Pb(NO3)2, and the plants were cultivated under controlled conditions for a period of six weeks. The impact of the lead treatments on the Pb content of the plants was found to be statistically significant at the 5% level. The highest Pb content (53.2 µg plant-1) was observed in the 400 mg kg-1 application of the transgenic plant. The results indicated that increasing doses of Pb led to reductions in the concentrations of N, P and K in both plant types in comparison with the control. The application of Pb at increasingly elevated doses to the soil led to a statistically significant elevation in the concentrations of both Cu and Zn in transgenic and non-transgenic tobacco plants, as compared to the concentrations in the control plants. The application of increasing doses of Pb to soil resulted in a statistically significant increase in the concentrations of Cu and Zn in transgenic and non-transgenic tobacco plants, when compared to the concentrations in the control plants. The effect of Pb applications on the concentrations of iron (Fe) and manganese (Mn) was found to be statistically insignificant. It was found that transgenic (p-cV-ChMTIIGFP) tobacco plants did not show toxic symptoms despite Pb stress and the reduction effect on nutrient uptake has the potential to increase the accumulation capacity by supporting fertilization.
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