In Vitro Propagation of Muscari (Muscari neglectum) Bulbs


Abstract views: 190 / PDF downloads: 130

Authors

DOI:

https://doi.org/10.5281/zenodo.7456312

Keywords:

Muscari, Muscari neglectum, in vitro, regeneration, micropropagation

Abstract

The study was conducted in the Tissue Culture Laboratory of the Field Crops Department of Agricultural Faculty of the Ege University in 2020-2021. Bulbs of Muscari neglectum collected from nature were used as genetic material in the study. These bulbs were cultured in nutrient media containing MS (Control) and MS+ TDZ (Thidiazuron) and BAP (6-Benzylaminopurine) 1.0; 2.0; 3.0; 4.0 for the regeneration. When the results were estimated; the highest means were obtained in MS+4.0 mg/l TDZ medium in terms of shoot number (8.2), shoot length (4.9 cm). MS+1.0 mg/l BAP medium had the highest means for root number (7.5), root length (3.3 cm). The shoots developed from these media were sub cultured in media containing MS+IBA for rooting. The media containing MS+2.0 mg/l IBA was found to be the highest in terms of plant number (8.3), plant height (8.7 cm) and root number (6.0). Muscari neglectum's bulbs have regeneration potential under in vitro conditions. The possibilities of using this plant as an ornamental or medicinal plant can be increased by using this technology

 

References

Acikgoz, N., Ilker, E., Gokcol, A., 2004. Evaluation of biological research in computer. E.U. TOTEM, Publication No:2, Izmir (in Turkish).

Açıkgöz, R. 2007. Türkiye’de Yayiliş Gösteren Endemik Muscari Aucheri (Boiss.) Baker Ve Muscari Discolor Boiss. & Hausskn Türlerinin Anatomik Özellikleri, Yüksek Lisans Tezi, Selçuk Üniversitesi Fen Bilimleri Enstitüsü, Biyoloji Ana Bilim Dalı, Konya.

Akyüz E. 2018. Bazı Frıtıllarıa türlerinde ın vıtro soğancık üretimi ve dış koşullara alıştırma çalışmaları. Hacettepe Üniversitesi Fen Bilimleri Enstitüsü. Biyoloji Ana Bilim Dalı Yüksek Lisans Tezi. Ankara.

Azad, M.A.K., Amin, M.N. 2012. Effects of Hormonal and Basal Nutrient Medium on In vitro Regeneration of an Ornamental Plant – Muscari armeniacum Leichtlin. ex Baker, Plant Tissue Cult. & Biotech. 22(2): 113-126.

Babaoğlu. M.. Yorgancılar. M.. Akbudak. M.A. 2001. Doku kültürü: temel laboratuar teknikleri. Bitki Biyoteknolojisi I. (çev: Özcan. S.. Gürel. E.. Babaoğlu. M.). Selçuk Üniversitesi Vakfı Yayınları. Konya.

Dane, F. 2006. Cytological and Histological Studies on Repruductive System of Hexaploid Bellevalia edirnensis Özhatay&Mathew (Hyacinthaceae), Acta Biologica Hungarica 57 (3): 339-354.

Dahlgren, R.M., Clifford, H.T., Yeo, P.F. 1985. The Families of The Monocotyledons; Structure, Evolution and Taxonomy (Academic Press: London). Doryanthaceae Liliaceae Lilidae Phormiaceae Thismiaceae Magnoliopsida.

Dalgıç, G. 1990. Edirne ve Kırklareli Bölgesi Hyacinthaceae Familyası Üzerinde Sitotaksonomik Araştırmalar Doktora Tezi, Trakya Üniversitesi Fen-Edebiyat Fakültesi Biyoloji Bölümü, Edirne.

Davis, P.H. 1984. Flora of Turkey and the East Aegean Islands. Vol. 8. Edinburgh: Edinburgh Univ. Press.

Davis, P.H., Stuart, D.C. 1984. Muscari, In Flora of Turkey and the East Eagean Islands. Ed.: Davis, P.H. Edinburgh, 8: 227-245.

Debergh. P.C., Zimmerman. R.H. 1993. Micropropagation technology and application. Kluwer Academic Publishers. Natherlands. 484 p.

Faruq, M. O., Shahinozzaman, M., Azad, M.A.K., Amin M.N. 2018. In vitro propagation of a cut flower variety Muscari armeniacum Leichtlin ex Baker through direct bulblet proliferation pathways, GSC Biological and Pharmaceutical Sciences, 5(1):67–75.

Gürsoy, M. 2016. Muscari Mirum Speta (Asparagaceae) ve Yakin Akrabalari (Muscari Massayanum Grunert, Muscari Tenuiflorum Tausch ve Muscari Latifolium Kirk)'Nin Morfolojik, Anatomik ve Ekolojik Özellikleri, Doktora Tezi, Manisa Celal Bayar Üniversitesi Fen Bilimleri Enstitüsü, Biyoloji Ana Bilim Dalı, Manisa.

Hazarika. B.N. 2006. Morpho-physiological disorders in in vitro culture of plants. Scientia Horticulturae 108 (2006): 105–120.

Karaoğlu C. 2004. Göl Soğanı (Leucojum aestivum L.)’nin In Vitro Koşullarında Hızlı Çoğaltımı, Yüksek Lisans Tezi, Ankara Üniversitesi Fen Bilimleri Enstitüsü, Tarla Bitkileri Bölümü, Ankara.

Koşanay, G. 2012. Farkli Populasyonlarda Yetişen Muscari Neglectum Guss. Türü Üzerinde Morfolojik, Anatomik ve Karyolojik Araştirmalar, Yüksek Lisans Tezi, Trakya Üniversitesi Fen Bilimleri Enstitüs, Fen-Edebiyat Fakültesi Biyoloji Bölümü, Edirne.

Murashige, T., Skoog, F. 1962, A revised medium for rapid growth and bioassay with tobacco cultures, Physiol. Plant., 15:473-479.

Özel, Ç.A., Khawar, K.M., Ünal, F. 2007. In vitro axillary bulblet regeneration of Turkish yellow grape Hyacinth, (Muscari macrocarpum Sweet) from twin scale explants. Research Journal of Agriculture and Biological Sciences, 3: 924-929.

Özel, Ç.A., Ünal F. 2016. Efficient in vitro Clonal Propagation of Muscari neglectum Guss. Ex. Ten Using Thidiazuron- α Naphthalene Acetic Acid, Turkish Journal of Agriculture - Food Science and Technology, 4(12):1173-1178.

Özel, Ç.A., Ünal, F. 2021. In vitro mass propagation of Muscari neglectum Guss. Ex. Ten., MAS Journal of Applied Sciences 6 (Özel Sayı): 1091–1102.

Ozturk, G. 2021a. In Vitro Propagation of Allium Neapolitanum, 29th of October symposium on scientific researches, Ankara, 293-296.

Ozturk, G. 2021b. In Vitro Regeneratıon of Snowdrop (Galanthus woronowoii), MAS Journal of Applied Sciences 6(4): 1027–1033.

Ozturk, G. 2021c. In Vıtro Regeneratıon of Tulıp (Tulipa L.), Astana Internatıonal Conference On Scıentıfıc Research October 23-24, Nur-Sultan, Kazakhstan, 164-168.

Steel, R.G.D., J.H. Torrie. 1980. Principles and Procedures of Statistics, McGaw-Hill Book Company, Inc. N.Y.

Stuart, D.C. 1970. Chromosome numbers in the genus Muscari Mill. Notes Roy. Bot. Garden Edinb. 30(1): 189-196.

Suzuki, S., Nakano, M. 2001. Organogenesis and somatic embryogenesis from callus cultures in Muscari armeniacum Leichtl. Ex. Bak. In Vitro Cellular and Developmental Biology, 37: 382-387.

Uranbey, S., Parmaksız, İ., Çöçü, S., Özcan, S., Mirici, S., Sancak, C., Sarıhan, E.O., İpek, A., Kaya, D., Gürbüz, B., Arslan, N., Khawar, K.M. 2006. In vitro micropropagation of endemic and endangered Muscari muscarimi. Agricultural Constraints in the Soil-Plant Atmosphere Continuum Proceedings of the International Symposium, Ghent, Belgium, 297- 300.

Uranbey, S. 2010. Stımulatıng effects of dıfferent basal medıa and cytokınıne types on regeneratıon of endemıc and endangered Muscarı aucherı, Arch. Biol. Sci., Belgrade, 62(3): 663-667.

Uysal, İ. 2002. Stachys cretica L. subsp. smyrnaea Rech Fil. endemik taksonunun morfolojisi, anatomisi ve ekolojisi üzerinde araştırmalar. Ekoloji Dergisi, 11(42):16-20.

Topal, A., Palabaş Uzun, S., Uzun, A. 2022. Mersin ili geofit bitki zenginliği. Turkish Journal of Forest Science, 6(1): 229.254.

Published

2022-12-23

How to Cite

ÖZTÜRK, G. (2022). In Vitro Propagation of Muscari (Muscari neglectum) Bulbs. MAS Journal of Applied Sciences, 7(Özel Sayı), 1160–1170. https://doi.org/10.5281/zenodo.7456312

Issue

Section

Articles