Determining the Effect of Different EMS (Ethyl Methane Sulphonate) Doses on Safflower

Abstract

The basic principle in mutation breeding is to identify and select the ones that are suitable for the purpose among the negative and positive variations that will occur with different mutagen doses applied by different methods. Creating hereditary differences that will increase variation in desired qualities depends on the selection of appropriate chemicals, their use in certain doses, and their effectiveness. This study was conducted in Dicle University Faculty of Agriculture Biotechnology laboratory and greenhouse conditions. The aim of the study is to determine the effect of different EMS doses on the properties examined in safflower. Seeds were treated with 0 (control), 10, 20, 30, 40, 50, 60, 70, 80, 90, 100 mM EMS. Seeds treated with EMS were subjected to a germination trial in 4 replications according to the random plot trial plan, with 20 seeds in each petri dish. Under greenhouse conditions, 20 seeds were sown in viols in 4 replicates and characteristics such as emergence rate, root length, seedling height, first leaf length, seedling fresh and dry weight were examined. As a result of the examined features, it was determined that there was a significant difference between the doses, and it was concluded that the highest values in all features were in the control group. It has been reported that increasing EMS doses cause a decrease in the examined property values, and the lowest values and deaths are obtained from the maximum dose of 100mM.