Determination and Prevalence of Some Important Pathogens in Newborn Calf Diarrhea in Bingol and its Surroundings
Abstract views: 296
/ 
PDF downloads: 142
DOI:
https://doi.org/10.5281/zenodo.8397035Keywords:
Calf, neonatal, diarrhea, gaita, test, PCR, infectionAbstract
The aim of this study was to determine clinically the prevalence of the most common pathogens causing diarrhea in 120 calves with diarrhea from 0 to 30 days old by rapid diagnostic test (HTT), standard diagnostic methods, and RT-PCR testing. The animal material consisted of diarrheal calves raised in Bingol and its surroundings. Therefore, the infectious agents causing calf diarrhea are proportionally eimeria 20.83%, escherichia coli 17.50%, rotavirus 16.81%, clostridium perfringens 13.27%, cryptosporidium spp 11.67%, coronavirus 9.16% and giardia 3.33%. Mixed cases have also been found. The same is true for the comparison of detection methods, rapid diagnostic tests for rotavirus (17/24-70.83%), coronavirus (7/24-29.16%), molecular analysis rotavirus (20/24-83.33%), coronavirus (11/24-45.83%) diarrhea cases. There was a slight numerical difference between the diagnosis of HTT (12/29-41.38%) and the microscopic diagnosis of cryptosporidium, one of the parasitic factors (14/29-48.27%), but there was no statistical significance (p>0.05). Hence, the diagnostic rate of the rapid detection kit is relatively high. Therefore, it was concluded that understanding the prevalence of identified pathogens and the differences between detection techniques would have important consequences for academic and clinical veterinarians in the field.
References
Akyüz, E., Naseri, A., Erkılıç, E.E., Makav, M., Uzlu, E., Kırmızıgül, A.H., Gökce, G., 2017. Neonatal buzağı ishalleri ve sepsis. Kafkas Üniversitesi Fen Bilimleri Enstitüsü Dergisi, 10 (2): 181-91.
Al M., Balıkçı E., 2012. Neonatal ishalli buzağılarda rotavirus, coronavirus, E. coli K99 ve cryptosporidium parvum'un hızlı test kitleri ile teşhisi ve enteropatojen ile maternal immünite ilişkisi. Fırat Üniversitesi Sağlık Bilimleri Veteriner Dergisi, 26(2): 73-78.
Albay, M., Şahinduran, Ş., Adanır, R., Yukarı, B., Köse, O., 2011. Efficacy of Albendazole and Two Different Doses of Paromomycin for Treatment of Naturally Occurring Giardia Infection in Lambs. Kafkas Üniversitesi Veteriner Fakültesi Dergisi, 17(6): 1021- 1024.
Alkan, F., 1998. Buzağı ishallerinde rotavirus ve coronavirusların rolü. Ankara Üniversitesi Veteriner Fakültesi Dergisi, 45: 29-37.
Altuğ, N., Yüksek, N., Özkan, C., Keleş, İ., Başbuğan, Y., Ağaoğlu, Z.T., Kaya, A., Akgül, Y., 2013. Neonatalbuzağı ishallerinin immunokromotografik test kitleri ile hızlı etiyolojik teşhisi. Yüzüncü Yıl Üniversitesi Veteriner Fakültesi Dergisi, 24: 123-128.
Al-Yousif, Y., Anderson, J., Bergstrom, C.C., Kapil, S., 2002. Development, evaluation, andapplication of lateral - flowimmunoassay(immunochromatography) fordetection of rotavirus in bovine fecal samples. Clinical and Diagnostic Laboratory Immunology, 9: 723–724.
Arslan, M.Ö., Kırmızıgül, A.H., Parmaksızoğlu, N., Erkılıç, E.E., 2015. Eimeria zuernii ile doğal enfekte buzağılarda kış coccidiosisi olgusu. Atatürk Üniversitesi Veteriner Bilimleri Dergisi, 10(3): 193-97.
Bal, D., 2019. Manisa yöresinde neonatal buzağı ishalleri üzerine etiyolojik araştırmalar. Yüksek lisans tezi. Kocatepe Üniversitesi Sağlık Bilimleri Enstitüsü, Afyonkarahisar.
Bilal, T., 2007.Yeni doğanların iç hastalıkları. İstanbul Üniversitesi; yayın no 4657. İstanbul.
Birdane, F.M., 2017. Çiftlik hayvanlarında kriptosporidiozis ishalleri. Kocatepe Veterinary Journal, 10: 91-98.
Cho, Y.I., Han, J.I., Wang, C., Cooper, V., Schwartz, K., Engelken, T., 2013. Case–control study of microbiological etiology associated with calf diarrhea. Veterinary Microbiology, 166(3-4): 375-85.
Cho, Y.I., Kim, W.I., Liu, S., Kinyon, J.M., Yoon, K.J., 2010. Development of a panel of multiplex real-time polymerase chain reaction assays for simultaneous detection of major agents causing calf diarrhea in feces. Journal of Veterinary Diagnostic Investigation, 22(4): 509-17.
Cho, Y.I., Yoon, K.J., 2014. An overview of calf diarrhea-infectious etiology, diagnosis, and intervention. Journal of Veterinary Science, 15 (1): 1-17.
Cho, K.O., Hoet, A.E., Loerch, S.C., Wittum, T.E., Saif, L.J., 2001. Evaluation of concurrent shedding of bovine coronavirus via the respiratory tract and enteric route in feedlot cattle. American Journal of Veterinary Research, 62: 1436–1441.
Constable, P.D., 2004. Antimicrobial use in the treatment of calf diarrhea. Journal of Veterinary Internal Medicine, 18: 8-17.
Cornish, T.E., Van Olphen, A.L., Cavender, J.L., Edwards, J.M., Jaeger, P.T., Vieyra, L.L., 2005. Comparison of ear notch immunohistochemistry, ear notch antigen-capture ELISA, and buffy coat virus isolation for detection of calves persistently infected with bovine viral diarrhea virus. Journal of Veterinary Diagnostic Investigation, 17(2): 110-17.
Cruvinel, L.B., Ayres, H., Zapa, D.M.B., Nicaretta, J.E., Couto, L.F.M., Heller L.M., Bastos T.S.A., Cruz B.C., Soares V.E., Teixeira W.F., de Oliveira J.S., Fritzen J.T., Alfieri A.A., Freire RL, Lopes WDZ., 2020. Prevalence and risk factors for agents causing diarrhea (Coronavirus, Rotavirus, Eimeria spp., Cryptosporidium spp., and nematodes helminthes) according to age in dairy calves from Brazil. Tropical Animal Health and Production, 2020 Mar; 52(2): 777-791.
Çabalar, M., Kaya, A., Arslan, S., 2007. Yeni doğan buzağıların ishal olgularında rotavirus ve coronavirus araştırılması. Veteriner Bilimleri Dergisi, 23(3-4): 103-06.
Debelo, M., Abdela, H., Tesfaye, A., Tiruneh, A., Mekonnen, G., Asefa, Z., Moje, N., 2021. Prevalence of Bovine Rotavirus and Coronavirus in Neonatal Calves in Dairy Farms of Addis Ababa, Ethiopia: Preliminary Study. BioMed Research International, Nov. 9: 1-6.
Elitok, Ö.M., Elitok, B., 2016. Neonatal buzağı ishallerinin tedavi ve korunmasında kolostrum serumlarının parenteral uygulanması. Kocatepe Veterinary Journal, 9: 211-214.
Garcia, A., Ruiz-Santa-Quiteria, J.A., Orden, J.A., 2000. Rotavirus and concurrent infections with other enterapathogens in noenatal diarrheic dairy calves in Spain. Comparative Immunology, Microbiology and Infectious Diseases, 23: 175 – 83.
Hammer, H.F., Fine, K.D., Santa Ana, C.A., Porter, J.L. Schiller, L.R., Fordtran, J.S., 1990. Carbohydrate Malabsorption. Its Measurement and its Contribution to Diarrhea. The Journal of Clinical Investigation, 86:1936-1944.
Hopkins, B.A., Quigley, J.D., 1997. Effects of method of colostrum feding and colostrum supplementation on concentrations of ımmunoglobulin G in the serum of neonatal calves. Journal of Diary Science, 80: 979-983.
İçen, H., Arserim, N.B., Işık, N., Özkan, C., Kaya, A., 2013. Prevalence of Four Enteropathogens with Immunochromatographic Rapid Test in the Feces of Diarrheic Calves in East and Southeast of Turkey. Pakistan Veterinary Journal, 33: 496-499.
Iturriza-Gomara, M., Wong, C., Blome, D., Desselberger, U., Gray J., 2002. Molecular characterization of VP6 genes of human rotavirus isolates: Correlation of genogroups with subgroups and evidence of independent segregation. Journal of Virology, 76: 6596-6601.
Jay, A.P., Sıv, M., Alfred, C.O., 1981. Role of pH in Production of Hydrogen from Carbohydrates by Colonic Bacterial Flora. Journal of Clinical Investigation, 67: 643-650.
Kalkan, C., Toparlı, R., 2017. Veteriner hekimliği terimleri sözlüğü. Türk Dil Kurumu Yayınları, 2.
Koçhan, A., Şimşek, A., İpek, D., İçen, H., 2020. Bir Buzağıda Giardia Duodenalis Kaynaklı Şiddetli Kanlı İshal Olgusu. Dicle Üniversitesi Veteriner Fakültesi Dergisi, 13(2): 179- 182.
Kozat, S., 2018. Hypothermia in newborn calves. Journal of İstanbul Veterinary Sciences, 2(1): 30-37.
Kozat, S., 2019. Yenidoğan buzağılarda kolostrum yönetiminin önemi. Atatürk Üniversitesi Veteriner Bilimleri Dergisi, 14(3): 343-53.
Kozat, S, Tuncay, İ., 2018. Siirt yöresindeki yenidoğan ishalli buzağılarda Rotavirus, Coronavirus, Cryptosporidium spp, Escherichia coli K 99 ve Giardia lamblia etkenlerinin prevalansı. Van Veterinary Journal, 29(1), 17-22.
Kozat, S., Voyvoda, H., 2006. İshalli buzağılarda kristalloid (laktatli ringer) ve kolloidal + kristalloid (%6 dekstran- 70 + laktatlı ringer) infüzyon solüsyonlarinin rehidratasyon etkinliği. Van Sağlık Bilimleri Dergisi, 9 (1): 139-51.
Lorenz, I., Fagan, J., More, S.J., 2011. Calf health from birth to weaning II.management of diarrhoea pre-weaned calves. Irish Veterinary Journal, 64:1- 9.
Luginbühl, A., Reitt, K., Metzler, A., ve ark., 2005. Feldstudie zu Prävalenz und Diagnostik von Durchfallerregern beim neonaten Kalb im Einzugsgebiet einer schweizerischen Nutztierpraxis, Schweiz Arch Tierheilk, 6: 245–252.
Matte, J.J., Girard, C.L., 1982. SeoaneJR, Absorption of colostral immunoglobulin G in the newborn dairy calf. Journal of Diary Science, 65: 1765-1772.
Oda, K., Nishida,Y., 1990. Prevalenca and distribution of bovine coccidia in japan. Japan Journal of Veterinary Science, 52:71-77.
Oku, Y., Kamiya, K., Kamiya, H., ve ark., 2001. Development of oligonucleotidelateral-flowimmunoassayformulti-parameterdetection. Journal of Immunological Methods, 258: 73–84.
Özkan, M., Gıcık, Y., Metin, H., Sarı, B., 2001. Prevence of cryptosporidium spp oocysts in diarrhoeic calves in Kars Province, Turkey. Turkish Journal of Veterinary Animal Science, 25: 161-64.
Smith, G.W., 2009. Treatment of calf diarrhea : oral fluid therapy. Veterinary Clinical North American Food Animal Practice, 25(1):55-72.
Şahal, M., Kurtdede, A., Börkü M.K., Ünsüren, H., İmren, H.Y., Özlem, M.B., Kalınbacak, A., 1994. Yeni doğan ishalli buzağıların klinik bulguları ve asit-baz dengesi dikkate alınarak sodyum bikarbonat ve elektrolitik sıvılarla sağaltımı. Ankara Üniversitesi Veteriner Fakültesi Dergisi, 41: 509-525.
Şahal, M., Terzi, O.S., Ceylan, E., Kara, E., 2018. Buzağı İshalleri ve Korunma Yöntemleri. Derleme/ Review Article, Lalahan Hayvancılık Araştırma Enstitüsü Dergisi, 58 (Özel Sayı): 41-49.
Şen, İ., Güzelbekteş, H., Yıldız, R., 2013. Neonatal buzağı ishalleri: patofizyoloji, epidemiyoloji, klinik, tedavi ve koruma. Türkiye Klinikleri Journal of Veterinary Sciences, 4:171-178.
Şentürk, S., 2001. Buzağı ishallerinde sıvı tedavisi. Journal of Research in Veterinary Medicine, 20: 161-167.
Şentürk, S., 2018. Olgu tartışmalı buzağı iç hastalıkları, Genişletilmiş kitap, 3. baskı. Özhan Matbaası, Bursa.
Downloads
Published
How to Cite
Issue
Section
License
Copyright (c) 2023 MAS Journal of Applied Sciences
This work is licensed under a Creative Commons Attribution-NonCommercial 4.0 International License.
The journal is licensed under a 