Open Access

In Vitro Propagation of Muscari (Muscari neglectum) Bulbs

Ege Üniversitesi Ziraat Fakültesi Tarla Bitkileri Bölümü, İzmir

Abstract

The study was conducted in the Tissue Culture Laboratory of the Field Crops Department of Agricultural Faculty of the Ege University in 2020-2021. Bulbs of Muscari neglectum collected from nature were used as genetic material in the study. These bulbs were cultured in nutrient media containing MS (Control) and MS+ TDZ (Thidiazuron) and BAP (6-Benzylaminopurine) 1.0; 2.0; 3.0; 4.0 for the regeneration. When the results were estimated; the highest means were obtained in MS+4.0 mg/l TDZ medium in terms of shoot number (8.2), shoot length (4.9 cm). MS+1.0 mg/l BAP medium had the highest means for root number (7.5), root length (3.3 cm). The shoots developed from these media were sub cultured in media containing MS+IBA for rooting. The media containing MS+2.0 mg/l IBA was found to be the highest in terms of plant number (8.3), plant height (8.7 cm) and root number (6.0). Muscari neglectum's bulbs have regeneration potential under in vitro conditions. The possibilities of using this plant as an ornamental or medicinal plant can be increased by using this technology

 

Keywords

How to Cite

ÖZTÜRK, G. (2022). In Vitro Propagation of Muscari (Muscari neglectum) Bulbs. MAS Journal of Applied Sciences, 7(Özel Sayı), 1160–1170. https://doi.org/10.5281/zenodo.7456312

References

📄 Acikgoz, N., Ilker, E., Gokcol, A., 2004. Evaluation of biological research in computer. E.U. TOTEM, Publication No:2, Izmir (in Turkish).
📄 Açıkgöz, R. 2007. Türkiye’de Yayiliş Gösteren Endemik Muscari Aucheri (Boiss.) Baker Ve Muscari Discolor Boiss. & Hausskn Türlerinin Anatomik Özellikleri, Yüksek Lisans Tezi, Selçuk Üniversitesi Fen Bilimleri Enstitüsü, Biyoloji Ana Bilim Dalı, Konya.
📄 Akyüz E. 2018. Bazı Frıtıllarıa türlerinde ın vıtro soğancık üretimi ve dış koşullara alıştırma çalışmaları. Hacettepe Üniversitesi Fen Bilimleri Enstitüsü. Biyoloji Ana Bilim Dalı Yüksek Lisans Tezi. Ankara.
📄 Azad, M.A.K., Amin, M.N. 2012. Effects of Hormonal and Basal Nutrient Medium on In vitro Regeneration of an Ornamental Plant – Muscari armeniacum Leichtlin. ex Baker, Plant Tissue Cult. & Biotech. 22(2): 113-126.
📄 Babaoğlu. M.. Yorgancılar. M.. Akbudak. M.A. 2001. Doku kültürü: temel laboratuar teknikleri. Bitki Biyoteknolojisi I. (çev: Özcan. S.. Gürel. E.. Babaoğlu. M.). Selçuk Üniversitesi Vakfı Yayınları. Konya.