Hemşirelik Öğrencileri, Üniversite Öğrencileri ve Hastane Yüzeylerinden İzole Edilen Staphylococcus aureus Suşlarında eta, etb ve etd Eksfoliyatif Toksin Genlerinin Araştırılması

Hanifi KÖRKOCA; Sümeyra SAVAŞ; Alper KARAGÖZ; Yusuf ALAN; Güzel Nur YILDIZ

doi:10.5281/zenodo.7472613

Authors

Hanifi KÖRKOCA Niğde Ömer Halisdemir Üniversitesi Tıp Fakültesi, Tıbbi Mikrobiyoloji Ana Bilim Dalı, Niğde https://orcid.org/0000-0002-2944-1227
Sümeyra SAVAŞ Bandırma Onyedi Eylül Üniversitesi, Tıp Fakültesi, Tıbbi Mikrobiyoloji Ana Bilim Dalı, Bandırma https://orcid.org/0000-0001-5057-9178
Alper KARAGÖZ Bilecik Şeyh Edebali Üniversitesi, Tıp Fakültesi, Tıbbi Mikrobiyoloji Ana Bilim Dalı, Bilecik https://orcid.org/0000-0002-8178-223X
Yusuf ALAN Bitlis Eren Üniversitesi Sağlık Hizmetleri Meslek Yüksekokulu Tıbbi Hizmetler ve Teknikler Bölümü, Bitlis https://orcid.org/0000-0003-0007-0212
Güzel Nur YILDIZ Muş Alparslan Üniversitesi Sağlık Hizmetleri Meslek Yüksekokulu Tıbbi Hizmetler ve Teknikler Bölümü, Muş https://orcid.org/0000-0003-1626-4089

DOI:

https://doi.org/10.5281/zenodo.7472613

Keywords:

Nursing students, University Students, Hospital surfaces, S. aureus, Exfoliative toxin gene

Abstract

The aim of this study was to investigate the presence of exfoliative toxin genes (eta, etb, etd) in S. aureus strains isolated from nursing students, university students and hospital surfaces. A total of 50 S. aureus strains isolated from 20 nursing students, 17 university students and 13 hospital surfaces were used in this study. The presence of exfoliative toxin (ET) genes (eta, etb and etd) was investigated by PCR. PFGE was used to investigate the clonal relationship of ET gene detected isolates. While etd gene was detected in 6% (3/50) in our study, eta and etb genes could not be detected. Two of the isolates with the etd gene were nursing students and one was a university student isolate. No ET gene was detected in any of the hospital surface isolates. It was determined that the isolates with etd gene detected were clonal related. Since S. aureus strains carrying exfoliative genes cause both community and healthcare-associated infections, comprehensive studies are needed to prevent infections that may arise from such strains.

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Investigation of eta, etb and etd Exfoliative Toxin Genes in Staphylococcus aureus Strains Isolated from Nursing Students, University Students and Hospital Surfaces

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