Shoot Regeneration of Sweet Potato [Ipomoea batatas (L.) Lam.] under In Vitro Conditons
Abstract views: 22 / PDF downloads: 10
DOI:
https://doi.org/10.5281/zenodo.14352745Keywords:
Sweet Potato [Ipomoea batatas (L.) Lam.], shoot initiation, Kinetin, IBA, NAAAbstract
Sweet potato [Ipomoea batatas (L.) Lam.] is a high income industrial plant used in human and animal nutrition and starch industry. Shoot initiation of axillary buds of sweet potato was investigated in vitro on different culture media in this study. Different concentrations of Kinetin were supplemented with MS basic medium and in vitro shoot regeneration was compared. The length of plantlets, root number, root length (cm) and number of leaves had the highest mean in 0.5 mg l-1 Kinetin medium with 8.0 cm, 3.0, 2.1 cm and 10.0, respectively. The growing shoots were sub-cultured in 1.0 mg l-1 IBA (Indole 3-butyric acid) and 1.0 mg l-1 NAA (1-Naphthalenetic acid) media and propagation was continued. The medium of 1.0 mg l-1 IBA had found to be successful in terms of plant height, root number, root length, leaf number and internode. In result, in vitro shoot regeneration of sweet potato [Ipomoea batatas (L.) Lam.] genotype has been found successful. So in vitro propagation can be done with axillary buds and a potential can be achieved in commercial production with sub-cultures.
References
Acikgöz, N., Ilker, E., Gokcol, A., 2004. Evaluation of biological research in computer. E.U. TOTEM, Publication No:2, Izmir.
Aka Kaçar, Y., Yalçın Mendi, Y., Yılmaz, N., Küden, A., Çetiner, S., 2001. In vitro besi ortamında kullanılan değişik katılaştırıcıları maddelerinin ve farklı pH düzeylerinin bazı kiraz (Prunus avium L.) anaçlarının çoğaltılması üzerine etkileri. I. Sert Çekirdekli Meyveler Sempozyumu, 25-28 Eylül, Yalova, s. 161-166.
Alula K., Zeleke H., Manikandan M., 2018. In vitro propagation of sweet potato (Ipomoea batatas (L.) Lam) through apical meristem culture. Journal of Pharmacognosy and Phytochemistry. 7(1):2386-2389.
An, L.V., Lindberg, J.E., 2004. Ensiling of sweet potato leaves (Ipomoea batatas (L.) Lam) and nutritive value of sweet potato leaf silage for growing pigs, Asian-Aus. Journal of Animal Science, 17:497-503.
Arıoğlu, H.H., 1997. Nişasta ve Şeker Bitkileri, Çukurova Üniversitesi Ziraat Fakültesi, Genel Yayın Sayı: 188.
Austin, A.D., 1988. The taxonomy, evolution and genetic diversity of sweetpotatoes and related wild species. In: International Potato Centre (CIP). Exploration, Maintenance and Utilization of Sweetpotato Genetic resources, pp. 27-59.
Baydemir, G., 2021. Portekiz Gandera yerel tatlı patates (Ipomoea batatas (L.) Lam) genotipinin in vitro mikro çoğaltılması üzerine bitki büyüme düzenleyicilerinin ve besi ortamlarının etkisi. Yüksek Lisans Tezi, Dicle Üniversitesi Bahçe Bitkileri Bölümü, Diyarbakır.
DoliĔski, R., Olek, A., 2013. Micropropagation of sweet potato (Ipomoea batatas (L.) Lam.) from node explants. Acta Scientiarum Polonorum Hortorum Cultus, 12(4): 117-127.
Dugassa, G., Feyissa, T., 2011. In Vıtro productıon of vırus-free sweet potato [Ipomoea batatas (L.) Lam] by merıstem culture and thermotherapy. Ethiopian Journal of Science and Technology, 34(1): 17–28.
Ferreira, M.E., 2021. Boas Praticas na Cultura da Batata-Doce. (www.iniav.pt), (Erişim Tarihi:18.06.2024).
Gaspar, T., Kevers, C., Penel, C., Greppin, H., Reid, D.M., Thorpe, T.A., 1996. Plant hormones and plant growth regulators in plant tissue culture. In Vitro Cellular & Developmental Biology-Plant, 32(4):272-289.
Grossmann, K., 2007. Auxin herbicide action: lifting the veil step by step. Plant Signaling & Behavior, 2:421-425.
Guillermo, E.D.P., Consuelo, R.I., Jorge, C.C., En IS, F., Walter, H., 2017. Development and agronomic evaluation of ın vitro somaclonal variation in sweet potato regenerated plants from direct organogenesis of roots. Asian Journal of Plant Science and Research, 7(1):39-44.
Hirai, D., Sakai, A., 2002 Simplified cryopreservation of sweet potato [Ipomoea batatas (L.) Lam.] by optimizing conditions for osmoprotection. Cell Biology and Morphogenesıs, 21:961–966.
İşler, N., 2009. Tatlı patates, (www.mku.edu.tr/files/898-beb83317-d033-46c0-8c9d- 243289a43abc.pdf.) (Erişim Tarihi: 18 Ekim 2024)
Kalashnikova, E.A., Kirakosyan, R.N., 2016. Modern aspects of biotechnology, M.: RGAU-MSKhA
Kalashnikova, E.A., 2020. Plant Cell Engineering: Textbook and Workshop, M.: Yurait, Ser. 76 Higher Education (2nd ed.).
Karan, Y.B, Şanli, Ö.G., 2021. The assessment of yield and quality traits of sweet potato (Ipomoea batatas L.) genotypes in middle Black Sea region, Turkey. PLoS One, 16(9): e0257703.
Kohlmeier, L., Hastings, S.B., 1995. Epidemiologic evidence of a role of carotenoids in cardiovascular disease prevention. The American Journal of Clinical Nutrition, 62(6):1370S-1376S.
Litz, R.E., Conover, R.A., 1978. In vitro propagation on sweet potato. HortScience, 13(6): 659–660.
Masekesa, R.T., Gasura, E., Matikiti, A., Kujeke, G., Ngadze, E., Icishahayo, D., Robertson, A., 2016. Effect of BAP, NAA and GA3, either alone or in combination, on meristem culture and plantlet establishment ın sweet potato (CV BRONDAL). African Journal of Food, Agriculture, Nutrition and Development, 16(1): 10653–10669.
Mukherjee, A., 2002. Effect on NaCl on in vitro propagation of sweet potato (Ipomoea batatas L.). Applied Biochemistry and Biotechnology, 102: 431–441.
Murashige, T., Skoog, F., 1962. A revised medium for rapid growth and bioassay with tobacco cultures. Plant Physiology, 15:473-479.
Ogero, K.O., Mwangi, M., Mburugu, G.N., Ngugi, M.M., Ombori, O., 2012. Low cost tissue culture technology in the regeneration of sweet potato (Ipomoea batatas (L) Lam). Research Journal of Biology, 2(2):51-58.
Ozturk, G., 2021. Field performances of different seedling types used in sweet potato [Ipomea batatas (L.) Lam] growing. Turkish Journal of Field Crops, 26(1): 54-59.
Ozturk, G., 2023. In Vitro Propagation of Ornothogalum umbellatum. ISPEC Journal of Agricultural Sciences, 7(4):825-832.
Parvin, J., Robbani, M., Hasan, M.F., Hoque, F., 2018. Standardization of plant growth regulators for successful tissue culture of sweet potato. Journal of the Bangladesh Agricultural University, 16(2):178-181.
Paula, M.A., Reis, V.M., Döbereiner, J., 1991. Interactions of Glomus clarum with Acetobacter diazotrophicus in infection of sweet potato (Ipomoea batatas), sugarcane (Saccharum spp.), and sweet sorghum (Sorghum vulgate). Biology and Fertility of Soils, 11:111–115.
Peters, D., Tinh, N.T., Thach, P.N., 2009. Sweet potatoroot silage fermentation and guality, Svedish University of Agricultural Sciences, Deparment of Animal Nutrition and ManagementUppsala, 46p.
Russell, R.M., 1998. Physiological and clinical significance of carotenoids. International Journal of Vitamin and Nutrition Research, 68: 349-353.
Scalbert, A., Manach, C., Morand, C., Remesy, C., Jimenez, L., 2005. Dietary polyphenols and the prevention of diseases. Critical Reviews in Food Science and Nutrition, 45: 287–306.
Scott, G.J., Otieno, J., Ferris, S.B., Muganga, A.K., Maldonado, L., 1998. Sweetpotato in Ugandan food systems: Enhancing food security and alleviating poverty. Program Report 1997-98, International Potato Center, Lima, Peru.
Shaibu, A.S., Abubakar, A.S., Lawan, Z. M., Ibrahim, A.K., Rabiu, H.M., Muhammad, A.I., 2016. Media optimization and effect of surface sterilization timing on ın vitro propagation of sweet potato. Proceedings of the 2nd International Conference on Drylands.
Shiotani, I., Huang, Z.Z., Sakamoto, S., Miyazaki, T., 1991. The role of wild Ipomoea trifida germplasm in sweet potato breeding. Proc. of the 9 th Symposium of the International Society for Tropical Root Crops, 20-26 October, Accra, Ghana.
Sivparsad, B.J., Gubba, A., 2012. Development of an efficient plant regeneration protocol for sweet potato (Ipomoea batatas L.) cv. Blesbok. African Journal of Biotechnology, 11(84): 14982-14987.
Steel, R.G.D., Torrie, J.H., 1980. Principles and procedures of statistics, McGaw-Hill Book Company, Inc. N.Y.
Şanlı, O.G., 2019. Bazı tatlı patates yerel genotiplerinin Tokat-Kazova şartlarında yetiştirilerek bitki gelişim özellikleri ve verim değerlerinin belirlenmesi. Yüksek Lisans Tezi, Tokat Gaziosmanpaşa Üniversitesi Fen Bilimleri Enstitüsü Tokat.
Templeton-Somers, K.M., Collins, W.W., 1985. Heritability of regeneration in tissue cultures of sweet potato (Ipomoea batatas L.). Theoretical and Applied Genetics, 71:835-841.
Tokusoglu, O., Kocak, S., Aycan, S., Yıldırım, Z., 2003. Comparative study for detection of B-group vitamins and folic acid by gas chromatograpy-mass spectrometry (GC-MS) and differential pulse polarography (DPP) in sweet potato (Ipomea batatas L.). In 2003 IFT Annual Meeting Book of Abstracts. p.239. July 12-16 in McCormick Place, South Building, Chicago IL, USA.
Tokusoglu, O., Yıldırım, Z., Durucasu, I., 2005. Nutraceutical phenolics (total polyphenols, chlorogenic [5-O-Caffeoylquinic] acid) in tubers, leaves, stalks and stems of new developed sweetpotato (Ipomea Batatas L.): Alterations in tubers during short-term storage. Journal of Food Technology, 3(3): 444-448.
Valverde, R.A., Clark, C.A., Valkonen, J.P.T., 2007. Viruses and virus disease compluxes of sweetpotato. Plant Viruses 1: 116-126.
Van Popoel, G., Goldbohm, R.A., 1995. Epidemiological evidence for β-carotene and cancer prevention. American Journal of Clinical Nutrition, 62: 1393-1402.
Vural, H., Eşiyok, D., Duman, İ., 2000. Kültür Sebzeleri (Sebze Yetiştirme), Ege Üniversitesi Basımevi, S:253-260
Woolfe, J.A., 1992. Sweet potato: an untapped food resource, Cambridge University Press, Cambridge, UK, 634s.
Ye, A., Singh, J., Somaratne, G., Nau, F., Ferrua, M.J., Dupont, D., Singh, R.P., 2020. Role of biochemical and mechanical disintegration on β-carotene release from steamed and fried sweet potatoes during in vitro gastric digestion. Food Research International, 136: 1-8.
Yıldırım, Z., Tokuşoğlu, Ö., Aygün, H., 2005. Ege bölgesine uygun tatlı patates (Ipomoea batatas L.) genotiplerinin belirlenmesi, Proje Sonuç Raporu (TOGTAG-2957), Tübitak, Ankara.
Yıldırım, Z., Tokuşoğlu, Ö., Öztürk, G., Aygün, H., 2007. Ege Bölgesine uygun tatlı patates (Ipomoea batatas L.) genotiplerinin belirlenmesi, Türkiye 7. Tarla Bitkileri Kongresi 25-27 Haziran, Erzurum, s.450-453.
Yıldırım, Z., 2009. Tatlı patates yetiştiriciliği. Tarım Türk Dergisi, sayı 15:70-71.
Yıldırım, Z., Tokuşoğlu, Ö., Öztürk, G., 2011. Determination of sweet potato [Ipomoea batatas (L.) Lam.] genotypes suitable to the Aegean region of Turkey. Turkish Journal of Field Crops, 16(1): 48-53.
Zobayed, F.A., Zobayed, S.M.A., Kubota, C., Kozai, T., Hasegawa, O., 1999. Supporting material affects the growth and development of in vitro sweet potato plantlets cultured photoautotrophically. In Vitro Cellular & Developmental Biology – Plant, 35:470-74.
Downloads
Published
How to Cite
Issue
Section
License
Copyright (c) 2024 The copyright of the published article belongs to its author.
This work is licensed under a Creative Commons Attribution-NonCommercial 4.0 International License.