Investigation of Hemocyte Types in Lepisma saccharina


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Authors

DOI:

https://doi.org/10.5281/zenodo.14325733

Keywords:

Hemolymph, insect, prohemocyte, plasmatocyte, granulocyte, oenocytoid

Abstract

Hemocytes are specialized hemolymph cells found in insects, responsible for a variety of important physiological functions such as immunity, wound healing, and the regulation of the insect's circulatory system. The study of insect hemocytes has become increasingly important in recent years due to the emergence of insect-borne diseases and the need to develop new strategies for insect control. Understanding the biology of insect hemocytes has the potential to lead to the development of novel insecticides and strategies for controlling insect-borne diseases. The aim of this study was to examine the hemocytes in Lepisma saccharina to determine if there were any variations in the types of hemocytes present among ametabolous and holometabolous insect or in comparison to other arthropods. Hemolymph of L. saccharina collected and hemolymph smears were stained with Wright’s stain. In adult L. saccharina two hemocyte types, prohemocyte and plasmatocyte were observed. The hemocyte types were identified based on their size, presence or absence of granules, and the ratio of their nucleus to cytoplasm, using light microscopy.

References

Demirsoy, A., 2003. Yaşamın Temel Kuralları, Omurgasızlar/ Böcekler, Entomoloji, Cilt II/ Kısım II, 9. Baskı, Meteksan Matbaacılık, Ankara.

Devauchelle, G., 1971. Etude ultrastructurale des hemocytes du Coleoptere Melolontha melolontha (L.), Journal of Ultrastructure Research, 34: 492-516.

Evans, C.J., Hartenstein, V., Banerjee, U., 2003. Conserved mechanisms in Drosophila and Vertebrate Hematopoiesis. Developmental Cell, 5:673-690.

Gillespie, J.P, Kanost, M.R., Trenczek, T., 1997. Biological mediators of insect immunity. Annual Review of Entomology, 42:611-643.

Glaser, R.W., 1918. On the existence of immunity principles in insects. Psyche, 25:39-46.

Grimaldi, D., Engel, M.S., 2005. Evolution of the Insects. Cambridge University Press.

Gupta, A.P., 1979. Arthropod hemocytes and phylogeny, Arthropod Phylogeny, 669-735.

Hartenstein, V., 2006. Blood cells and blood cell development in the animal kingdom, Annual Review of Cell and Developmental Biology, 22:677-712.

Irving, P., Ubeda, J., Doucet, D., Troxler, L., Lagueux, M., Zachary, D., Hoffmann, J., Hetru, C., Meister, M., 2005. New insights into Drosophila larval haemocyte functions through genomewide analysis. Cellular Microbiology, 7:335–350.

Jalali, J., Salehi, R., 2008. The hemocyte types, differential and total count in Papilio demoleus L. (Lepidoptera: Papilionidae) during post-embryonic development. Munis Entomology & Zoology, 3(1):199-216.

Joshi, M.J., Prithiv Raj, V., Solanki, C.B., 2020. Silverfish (Lepisma saccharina): An overview and their management. Agriculture & Foods: e-Newsletter, 2.

Kwon, H., Bang, K., Cho, S., 2014. Characterization of the hemocytes in larvae of Protaetia brevitarsis seulensis: involvement of granulocyte-mediated phagocytosis. PLoS One, 9(8): e103620.

Lackie, A.M., 1988. Haemocyte behavior, Advances in Insect Physiology, 21:85-178.

Lan, W., Liu, S., Zhao, L., Su, Y., 2020. Regulation of Drosophila hematopoiesis in Lymph Gland: from a developmental signaling point of view. International Journal of Molecular Sciences, 21(15):52-46.

Lanot, R., Zachary, D., Holder, F., Meister, M., 2001. Postembryonic hematopoiesis in Drosophila. Developmental biology, 230(2):243-257.

Larsen, W.J., 1976. Cell remodeling in the fat body of an insect, Tissue Cell, 8(1):73-92.

Lavine, M.D., Strand, M.R., 2002. Insect hemocytes and their role in cellular immune responses. Insect Biochemistry and Molecular Biology, 32:1237-1242.

Levin, D.M., 2007. An integrin required fort he encapsulation immune response in the tobacco hornworm Manduca sexta L. (Lepidoptera: Sphingidae), Doktora Tezi, Kansas State University, College of Agriculture, Department of Entomology, Manhattan, Kansas.

Morley, EL., Jones, G., Radford, AN., 2014. The importance of invertebrateswhen considering the impacts of anthropo-genic noise. Proceedings of the Royal Society B, 281:20132683.

Müller, U., Vogel, P., Alber, G., Schaub, G.A., 2008. The innate immune system of mammals and insects. Trends in Innate Immunity, 15:21-44.

Owens, B., 2019. How plants and insects inherit immunity from their parents. Nature, 575(7784):S55-S56.

Öztürk, G., Akat Çömden, E., Arıkan, H., 2024. Characterization of hemocyte types in Bombyx mori throughout pupa, larva, and adult stages by light microscopy. Biharean Biologist, 18(1):12-18.

Punzo, F., 1987. Physiological amino acids in brain tissue of the lepismatids, Lepisma saccharina and Thermobia domestica (Insecta, Thysanura). Comparative Biochemistry and Physiology, Part C Comparative Pharmacology, 88(2):255-258.

Sheehan, G., Farrell, G., Kavanagh, K., 2020. Immune priming: the secret weapon of the insect world. Virulence, 11(1):238-246.

Silva, J.E.B., Boleli, I.C., Simões, Z.L.P., 2002. Hemocyte types and total and differential counts in unparasitized and parasitized Anastrepha obliqua (Diptera, Tephritidae) larvae. Brazilian Journal of Biology, 62: 689-699.

Stoepler, T.M., Castillo, J.C., Lill, J.T., Eleftherianos, I., 2012. A simple protocol for extracting hemocytes from wild caterpillars. Journal of Visualized Experiments, 69:1-6.

Strand, M.R., Pech, L.L., 1995. Immunological basis for compatibility in parasitoid–host relationships. Annual Review Entomology, 40:31-56.

Sułek, M., Kordaczuk, J., Wojda, I., 2021. Current understanding of immune priming phenomena in insects. Journal of Invertebrate Pathology,185:107656.

Trautwein, M.D., Wiegmann, B.M., Beutel, R., Kjer, K.M., Yeates, D.K., 2012. Advances in insect phylogeny at the dawn of the postgenomic era. Annual Review of Entomology, 57:449-68.

van Gelderen, F., 2010. Radiography of the Silverfish (Lepisma saccharina). Shadows: The New Zealand Journal of Medical Radiation Technology, 53(3):18.

Wu, G., Liu, Y., Ding, Y., Yi, Y., 2016. Ultrastructural and functional characterization of circulating hemocytes from Galleria mellonella larva: Cell types and their role in the innate immunity. Tissue and Cell, 48(4):297-304.

Yamashita, M., Iwabuchi, K., 2001. Bombyx mori prohemocyte division and differentiation in individual microcultures. Journal of Insect Physiology, 47(4-5):325-331.

Yelkovan, S., Arıkan, H., Çakıcı, Ö., 2020. Caste and age-related changes in circulatory hemocytes of honey bee, Apis mellifera anatolica (Hymenoptera: Apidae). Journal of Apicultural Research, 60(3):512–521.

Zhang K., Yu, S., Su, J., Xu, M., Tan, P., Zhang, Y., Xiang, Z. And Cui, H., 2015. Identification and characterization of three novel hemocyte-specific promoters in silkworm Bombyx mori. Biochemical and Biophysical Research Communications, 461(1):102‐108.

Zimian, D., Yonghua, Z., Xiwu, G., 1997. Medicinal insects in China. Ecology of Food and Nutrition, 36(2-4):209-220.

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Published

2024-12-22

How to Cite

ÖZTÜRK , G., ARIKAN , H., & ÖZTÜRK, G. (2024). Investigation of Hemocyte Types in Lepisma saccharina. MAS Journal of Applied Sciences, 9(4), 1040–1047. https://doi.org/10.5281/zenodo.14325733

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