In Vitro Micropropagation of Opuntia microdasys Albata

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Opuntia microdasys, tissue culture, succulent, opintia


Cacti are the Cactaceae family and are a desert plants adapted to arid regions. Cacti known as succulent plants and store water in their leaves. They have a stems in arid region and soil conditions. They are used as landscaping, ornamental plants and potted plants. In addition to the vitamins and amino acids they contain, also rich in secondary metabolites. This study was conducted in tissue culture propagation of Opuntia microdasys, is named as ‘tavşan kulaklı’ cactus. Succulent stems, called pads, were used as genetic material. Opuntia microdasys were cultured on Murashige and Skoog (1962) medium (MS) with TDZ and BAP (0.5 mg L-1; 1.0 mg L-1; 2.0 mg L-1; 3.0 mg L-1). Best rejeneration were obtained with TDZ and BAP at 0.5 mg L-1 as 3.5 for pad number. The media of 0.5 mg L-1 TDZ was found to be the highest with 1.4 cm in terms of pad length. On the other hand, 0.5 mg L-1 BAP medium had the highest mean for root number and root length, with 2.5 and 1.6 cm, respectively. It has been seen that the succulent variety Opuntia microdasys, not only use as an ornamental plant but also medicinal area. Opuntia microdasys has a potential to be evaluated under in vitro conditions. Therefore, this study can be developed using different nutrient media and growth regulation for shoot and root formation with following studies. So in vitro propagation for Opuntia microdasys can be an alternative to other production.



Acikgoz, N., Ilker, E., Gokcol, A., 2004. Evaluation of biological research in computer, E.U. TOTEM, Publication No:2, Izmir.

Ali A., Naz S., Ahmad Siddiqui F., Iqbal J., 2001. Callogenesis, embryogenesis and organogenesis in christmas cactus (Schlumbegera bridesi). Pakistan Journal of Botany, 33: 569-574.

Aliyu, B.S., Mustapha, Y., 2007. Effect of different media on the in vitro growth of cactus (Opuntia ficus-indica) explants. African Journal of Biotechnology, 6(11): 1330-1331.

Anderson, E.F., 2001. The cactus family. Timber Press, Oregon.

Angulo-Bejarano, P.I., Paredes-López O., 2011. Development of a regeneration protocol through indirect organogenesis in prickly pear cactus (Opuntia ficus-indica (L.) Mill). Scientia Horticulturae, 128: 283-288.

Arakaki, M., Christin, P.A., Nyffeler, R., Lendel, A., Eggli, U., Ogburn, R.M., Spriggs, E., Moore, M.J., Edwards, E.J., 2011. Contemporaneous and recent radiations of the world’s major succulent plant lineages. Proc Natl Acad Sci USA, 108:8379–8384.

Arellano-Perusquía, A., López-Peralta,M.C.G., Chablé-Moreno, F., Estrada-Luna, A.A., 2013. Effect of Growth Regulators on The Organogenesıs and Multiplication of Ortegocactus Macdougallıı Alexander, Propagation of Ornamental Plants, 13(4): 160-167.

Ault, J.R., Blackmon, W.J., 1987. In vitro propagation of Ferocactus acanthoides (Cactaceae). HortScience, 22: 126-127.

Ajose F.O., 2007. Some Nigerian plants of dermatologic importance, International Journal of Dermatology, 1:48-55.

Bouzroud, S., El Maaiden, E., Sobeh, M., Devkota, K.P., 2022. Micropropagation of Opuntia and Other Cacti Species Through Axillary Shoot Proliferation: A Comprehensive Review. Frontiers Plant Science, 13:1-12.

Cardarelli, M., Borgognone, D., Colla G., 2010. In vitro propagation of Obregonia denegrii Fri. (Cactaceae).Propagation of Ornamental Plants, 10: 29-36.

Copasso, F., Borrelli, F., Capasso, R., Di Carlo, G., Izzo, A.A., Pinto, L., 1998. Aloe and Its Therapeutic Use. Phytotherapy Research, 12: 124–127.

Dávila-Figueroa, C.A., De La Rosa-Carrillo, M.L., Pérez-Molphe-Balch, E., 2005. In vitro propagation of eight species or subspecies of Turbinicarpus (Cactaceae). in vitro Cellular & Developmental Biology-Plant, 41: 540-545.

El Finti, A., El Boullani, R., El Ayadi, F., Ait Aabd, N., El Mousadik, A., 2012. Micropropagation in vitro of Opuntia Ficus-Indica in south of Morocco. International Journal of Chemical and Biochemical Sciences, 1:6-10.

Estrada-Luna, A.A., Martínez-Hernández J.J., Torres-Torres, M.E., Chablé-Moreno, F., 2008. in vitro micropropagation of the ornamental prickly pear cactus Opuntia lanigera Salm-Dyck., and effects of sprayed GA3 after transplantation to ex vitro conditions. Scientia Horticulturae, 117: 378-385.

Estrada-Luna, A.A., López-Peralta, C., Cárdenas-Soriano, E., 2002. In vitro micrografting and the histology of graft union formation of selected species of prickly pear cactus (Opuntia spp.). Scientia Horticulturae, 92: 317-327.

García-Saucedo, P., Valdez-Morales, M., Valverde, M.E., Cruz-Hernández, A. and Paredes-López, O., 2005. Plant regeneration of three Opuntia genotypes used as human food. Plant Cell, Tissue and Organ Culture, 80: 215–219.

Gibson, A.C. and Nobel, P.S., 1986. The cactus primer. Harvard University Press, Cambridge.

Gómez-Juárez, J.L., Morales, J.E., Lechuga-Corchado, J.A., Cruz-Sosa, F., 2006. Reproducción in vitro del garambullo Myrtillocactus geometrizans (Martius) Console. Cactáceas y Suculentas Mexicanas, 51: 36-45.

Heikal, A., Marwa, E., Abd El-Sadek, A., Salama, H., Taha, S., 2021. Comparative study between in vivo- and in vitro-derived extracts of cactus (Opuntis ficus-indica L. Mill) against prostate and mammary cancer cell lines. Helıyon,

Hubstenberger, J.F., Clayton, P.W., Phillips, G.C., 1992. Micropropagation of cacti (Cactaceae). In: Biotechnology in Agriculture and Forestry. High tech and micropropagation IV. Vol. 20. Ed: Bajaj, Y.P.S. Springer-Verlag, Berlin International Horticultural Congress. pp 49– 68.

Johnson, J., Emino, E.R., 1979. Tissue culture propagation in the cactaceae. Cactus & Succulent Journal, 51:275–277.

Kumar, K., Singh, D., Singh, R.S., 2018. Cactus Pear: Cultivation and uses. Technical Bulletin No. 73.

Liu, P., Chen, D., Shi, J., 2013. Chemical constituents, biological activity and agricultural cultivation of Aloe vera, Asian Journal of Chemistry, 25(12): 6477–6485.

López-Palacios C., Reyes-AgUero, J.A., Peña-Valdivia, C.B., Aguirre- Rivera, J.R., 2019. Physical characteristics of fruits and seeds of Opuntia sp. an evidence of changes through domestication in theSouthern Mexican Plateau. Genetic Resources and Crop Evolution, 66:349–362.

López‑Palacios, C., Peña‑Valdivia, C.B., 2020. Screening of secondary metabolites in cladodes to further decode the domestication process in the genus Opuntia (Cactaceae). Planta, 251:74.

Marhri, A., Tikent, A., Garros, L., Merah, O., Elamrani, A., Hano, C., Abid, M., Addi, M., 2023. Rapid and Efficient In Vitro Propagation Protocol of Endangered Wild Prickly Pear Growing in Eastern Morocco. Horticulturae. 1-9.

Murashige, T., Skoog, F., 1962. A revised medium for rapid growth and bioassay with tobacco cultures, Physiologia Plantarum, 15:473-479.

Okcuoğlu, M.C., 2023. Aloe Vera jeli ile yenilenebilir film malzemelerin hazırlanması ve karakterizasyonu, Selçuk Üniversitesi Fen Bilimleri Enstitüsü Yüksek Lisans Tezi, Konya.

Özçatalbaş, O., Erdoğan, R., 2013. Akdeniz bahçeleri ve günümüz peyzaj tasarım çalışmalarına yansımaları. Peyzaj Mimarlığı 5. Kongresi, 14-17 Kasım 2013, Adana 834s.

Ozturk, G., 2021. In Vitro Regeneratıon of Tulip (Tulipa L.), Astana Internatıonal Conference On Scıentıfıc Research October 23-24, Nur-Sultan, Kazakhstan, p, 164-168.

Ozturk, G., 2022. In Vitro Propagation of Muscari (Muscari neglectum) Bulbs. MAS Journal of Applied Sciences, 7: 1160–1170.

Ozturk, G., 2023a. Micropropagation of Gasteria Okavango. 5. Internatıonal Scıentıfıc Research And Innovatıon Congress 20/21 May, Ankara / Turkey. pp.166-170.

Ozturk, G., 2023b. in vitro Regeneration of Gollum Jade Crassula. 5. Internatıonal Scıentıfıc Research And Innovatıon Congress 20/21 May, Ankara / Turkey. pp.171-175.

Smith, R.H., Burdick, J.P., Anthony, J. and Reilley, A.A., 1991. in vitro propagation of Coryphantha macromeris. Horticulture Science, 26(3): 315.

Steel, R.G.D., Torrie, J.H., 1980. Principles and Procedures of Statistics, McGaw-Hill Book Company, Inc. N.Y.

Stuppy, W., Nagl, W., 1992. Regeneration and propagation of Ariocarpus retusus Scheidw. (Cactaceae) via somatic embryogenesis. Bradleya, 10: 85-88.

Ramírez-Malagón, R., Aguilar-Ramírez, I., Borodanenko, A., Pérez-Moreno, L., Barrera-Guera J.L., Núñez-Palenius, H.G., Ochoa-Alejo, N., 2007. In vitro propagation of ten threatened species of Mammillaria (Cactaceae). In Vitro Cellular & Developmental Biology-Plant, 43: 660-665.

Rodriguez, O.J.L., Ramirez-Pantoja, P.E., 2020. Micropropagation of selected materials of Opuntia ficus indica L through culture in vitro of areola. Journal of Applied Biotechnology & Bioengineering, 7: 121–126.

Rojas-Aréchiga, M., Vásquez-Yanes, C., 2000. Cactus seed germination: a review. Journal of Arid Environments, 44:85–104.

Upton, R., Axentiev, P., Swisher, D., 2012. Aloe vera leaf, Aloe vera leaf juice, Aloe vera ınner leaf juice, In: Upton R. (Ed.), American Herbal Pharmacopoeia, California, 1–52.

Wyka, T.P., Hamerska, M., Wróblewska, M., 2006. Organogenesis of vegetative shoots from in vitro cultured flower buds of Mammillaria albicoma (Cactaceae). Plant Cell, Tissue and Organ Culture, 87: 27-32.



How to Cite

ÖZTÜRK, G. (2023). In Vitro Micropropagation of Opuntia microdasys Albata. MAS Journal of Applied Sciences, 8(Özel Sayı), 1069–1076.