Determination of Tolerances of Some Flax Varieties to Different Doses of Salt Concentrations in Early Development Period

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Flax, NaCl, germination seed, correlation, tolererence, proline, MDA


It is widely accepted that soil salinity increases over time and is triggered by the effect of climate change. The aim of this research is to examine the germination, growth response and tolerance of some flax (Linum usitatissimum L.) varieties of different salinity levels. This study was carried out with 11 flax genotypes in 2022 in the germination laboratory of Muş Alparslan University, Faculty of Applied Sciences, Plant Production and Technologies. The research was established according to the Randomized Plots Trial Design with 4 replications, and 0, 75, 150 and 225 mM NaCl salt solution levels were applied. In the analysis of variance, genotype, salt levels and genotype*salt level interaction were found to be statistically significant at the P <0.01 level in terms of all the traits examined. In all cultivars used in the study, 75 mM NaCl concentration showed a positive stimulating effect on germination. However, although 150 mM NaCl concentration had a germination-promoting effect, it negatively affected normal seedling formation. As a result, when the anatomical, physiological and biochemical findings of flax varieties are evaluated as a whole in the early development period; It was determined that the most sensitive cultivars to salinity were G2, G10, and G11 varieties, moderately sensitive varieties were G1, G4, G5 and G8, in terms of all doses, the most stable variety was G3, in addition G6, G7 and G9 of varieties showed good results in terms of all traits. However, it was concluded that these varieties should be cultivated under field conditions in order to obtain more decisive results.


Apel, K., Hirt, H., 2004, Reactive oxygen species: metabolism, oxidative stress and signal transduction, Annual Review of Plant Biology, 55: 373-399.

Asada, K., 1999, The water–water cycle in chloroplasts: scavenging of active oxygens and dissipation of excess photons, Annual Review of Plant Physiology and Plant Molecular Biology, 50: 601-39.

Ashkan, A., Jalal, M. 2013. Effects of salinity stress on seed germination and seedling vigor indices of two halophytic plant species (Agropyron elongatum and A. pectiniforme). International Journal of Agriculture and Crop Sciences, 5(22): 2669-2676.

Ashraf, M., 2004. Some important physiological selection criteria for salt tolerance in plants. Flora-Morphology, Distribution, Functional Ecology of Plants, 199(5): 361-376.

Assaha, D.V., Ueda, A., Saneoka, H., Al-Yahyai, R., Yaish, M.W., 2017. The role of Na+ and K+ transporters in salt stress adaptation in glycophytes. Frontiers in physiology, 8: 509.

Bates, L., Waldren, R., Teare, I., 1973. Rapid determination of free proline for water-stress studies. Plant Soil 39:189–198.

Bilmez-Özçınar, A., 2021, Yağlı tohumlu bitkilerin tuzluluğa toleransı, Kalkınma Odaklı Doğal Kaynak Temelli İnovasyon, Iksad Publications, Ankara, 27-40.

Chuamnakthong, S., Nampei, M., Ueda, A., 2019. Characterization of Na+ exclusion mechanism in rice under saline-alkaline stress conditions. Plant Science, 287: 110171.

Datta, K.S., Dayal, J., 1991. Studies on germination and early seedling growth of gram (Cicer arietinum L.) as affected by salinity. New Trends in Plant Physiology, 1: 273-276.

El-Bassiouny, H., Sadak, M.S., 2015. Impact of foliar application of ascorbic acid and α-tocopherol on antioxidant activity and some biochemical aspects of flax cultivars under salinity stress. Acta Biológica Colombiana, 20(2): 209-222.

El-Beltagi, H.S., Salama, Z.A., El Hariri, D. M., 2008. Some biochemical markers for evaluation of flax cultivars under salt stress conditions. Journal of Natural Fibers, 5(4): 316-330.

Ellis, R.H., Roberts, E.H., 1981. The quantification of ageing and survival in orthodox seeds. Seed Science and Technology (Netherlands).

FAOSTAT, 2020. Dünyada keten tohumu üretimi. #data/QCL/visualize (Erişim tarihi: 10.12.2022).

Fang, S., Wang, J., Wei, Z., Zhu, Z., 2006. Methods to break seed dormancy in Cyclocarya paliurus (Batal) Iljinskaja. Scientia Horticulturae, 110(3): 305-309.

Foti, C., Khah, E.M., Pavli, O.I., 2019. Germination profiling of lentil genotypes subjected to salinity stress. Plant Biology, 21(3): 480-486.

Hall, L.M., Booker, H., Siloto, R.M., Jhala, A.J., Weselake, R.J., 2016. Flax (Linum usitatissimum L.). In Industrial oil crops (pp. 157-194). AOCS Press.

Heath, R.L., Packer, L., 1968. Photoperoxidation in isolated chloroplasts: I. Kinetics and stoichiometry of fatty acid peroxidation. Archives of biochemistry and biophysics, 125(1): 189-198.

Houle, G., Morel, L., Reynolds, C.E., Siégel, J., 2001. The effect of salinity on different developmental stages of an endemic annual plant, Aster laurentianus (Asteraceae). American Journal of Botany, 88(1): 62-67.

Huang, J., Redmann, R.E., 1995. Salt tolerance of Hordeum and Brassica species during germination and early seedling growth. Canadian Journal of Plant Science, 75(4): 815-819.

Iqbal, M., Ashraf, M., Jamil, A., Ur‐Rehman, S., 2006. Does seed priming induce changes in the levels of some endogenous plant hormones in hexaploid wheat plants under salt stress? Journal of integrative plant Biology, 48(2): 181-189.

Jaleel, C.A., Gopi, R., Sankar, B., Manivannan, P., Kishorekumar, A., Sridharan, R., Panneerselvam, R., 2007. Studies on germination, seedling vigour, lipid peroxidation and proline metabolism in Catharanthus roseus seedlings under salt stress. South African Journal of Botany, 73(2): 190-195.

Khayamim, S., Tavkol Afshari, R., Sadeghian, S.Y., Poustini, K., Roozbeh, F., Abbasi, Z., 2014. Seed germination, plant establishment, and yield of sugar beet genotypes under salinity stress. Journal of Agricultural Science and Technology, 16(4): 779-790.

Koçak, M.Z., Göre, M., Kurt, O., 2022. The effect of different salinity levels on germination development of some flax (Linum usitatissimum L.) varieties. Turkish Journal of Agriculture-Food Science and Technology, 10(4): 657-662.

Lay, C.L., Dybing, C.D., Robbelen, G., Downey, R.K., Ashri, A., 1990. Oil crops of the world. McGraw-Hill.

Li, J., Yin, L.Y., Jongsma, M.A., Wang, C. Y., 2011. Effects of light, hydropriming and abiotic stress on seed germination, and shoot and root growth of pyrethrum (Tanacetum cinerariifolium). Industrial crops and products, 34(3): 1543-1549.

Maas, E.V., Nieman, R.H., 1978. Physiology of plant tolerance to salinity. Crop Tolerance To Suboptimal Land Conditions, 32: 277-299.

Misra, N., Dwivedi, U. N., 2004. Genotypic difference in salinity tolerance of green gram cultivars. Plant Science, 166(5): 1135-1142.

Moghaddam, M., Babaei, K., Saeedi Pooya, E., 2018. Germination and growth response of flax (Linum usitatissimum) to salinity stress by different salt types and concentrations. Journal of Plant Nutrition, 41(5): 563-573.

Pan, J., Li, Z., Dai, S., Ding, H., Wang, Q., Li, X., Liu, W., 2020. Integrative analyses of transcriptomics and metabolomics upon seed germination of foxtail millet in response to salinity. Scientific reports, 10(1): 1-16.

Parida, A.K., Das, A.B., 2005. Salt tolerance and salinity effects on plants: a review. Ecotoxicology And Environmental Safety, 60: 324-349.

Rajabi Dehnavi, A., Zahedi, M., Ludwiczak, A., Cardenas Perez, S., Piernik, A., 2020. Effect of salinity on seed germination and seedling development of sorghum (Sorghum bicolor L.) Moench) genotypes. Agronomy, 10(6), 859.

Saglam, A., Kadioglu, A., Demiralay, M., Terzi, R., 2014. Leaf rolling reduces photosynthetic loss in maize under severe drought. Acta Botanica Croatica, 73(2): 315-323.

Shahid, S.A., Zaman, M., Heng, L., 2018. Introduction to soil salinity, sodicity and diagnostics techniques. In Guideline for salinity assessment, mitigation and adaptation using nuclear and related techniques (pp. 1-42). Springer, Cham.

Shrivastava, P., Kumar, R., 2015. Soil salinity: A serious environmental issue and plant growth promoting bacteria as one of the tools for its alleviation. Saudi journal of biological sciences, 22(2): 123-131.

Song, J., Chen, M., Feng, G., Jia, Y., Wang, B., Zhang, F., 2009. Effect of salinity on growth, ion accumulation and the roles of ions in osmotic adjustment of two populations of Suaeda salsa. Plant and Soil, 314(1): 133-141.

Tobe, K., Li, X., Omasa, K., 2004. Effects of five different salts on seed germination and seedling growth of Haloxylon ammodendron (Chenopodiaceae). Seed Science Research, 14(4): 345-353.

Velikova, V., Yordanov, I., Edreva, A., 2000. Oxidative stress and some antioxidant systems in acid rain-treated bean plants, protective role of exogenous polyamines. Plant Sciences 151:59–66.

Vicente, O., Boscaiu, M., Naranjo, M.Á., Estrelles, E., Bellés, J.M., Soriano, P., 2004. Responses to salt stress in the halophyte Plantago crassifolia (Plantaginaceae). Journal of Arid Environments, 58(4): 463-481.

Werner, J.E., Finkelstein, R.R., 1995. Arabidopsis mutants with reduced response to NaCl and osmotic stress. Physiologia Plantarum, 93(4): 659-666.

Yamaguchi, T., Blumwald, E., 2005. Developing salt-tolerant crop plants: challenges and opportunities. Trends in plant science, 10(12): 615-620.

Yang, F., Chen, H., Liu, C., Li, L., Liu, L., Han, X., Sha, A., 2020. Transcriptome profile analysis of two Vicia faba cultivars with contrasting salinity tolerance during seed germination. Scientific reports, 10(1): 1-10.

Yokoi, S., Bressan, R.A. Hasegawa, P.M., 2002. Salt stress tolerance of plants, JIRCAS Working Report, 25-33.




How to Cite

YAŞAR, M., & YETİŞSİN, F. (2023). Determination of Tolerances of Some Flax Varieties to Different Doses of Salt Concentrations in Early Development Period. MAS Journal of Applied Sciences, 8(1), 144–157.