Eskisehir Osmangazi University, Faculty of Agriculture, Department of Food Engineering, Eskisehir, Turkey
Abstract
The propolis, which is a natural product that is collected by honey bees from the plants, especially flowers and buds, by mixing it with wax and resin, is used in the hive for many purposes. Apart from the use by the bees in the hive, propolis is a versatile compound that is used in medicine, cosmetics, food industry and apitherapy and which contains a wide variety of chemical substances having antibacterial and antioxidant effects. It was determined that the antimicrobial effects of propolis samples were higher against the Gram-positive bacteria species included in the study when compared to the Gram-negative bacteria and yeasts. The majority of the samples were found to be highly sensitive (16 mm and larger), while some were found to be moderately sensitive (11-15 mm). Among all strains of bacteria; it was demonstrated that the propolis sample obtained from Cankiri (Yaprakli) region performed the highest activity against Staphylococcus aureus while the lowest activity was performed by the propolis sample obtained from Balıkesir region against E. coli. In addition, it was determined that the propolis sample obtained from Eskişehir region had no antifungal effect against Candida parapsilosis yeast strain. It was specified that the antimicrobial activity demonstrated by all microorganisms against the propolis samples was less effective when compared to the control compounds, except for Candida krusei.
GÜLBANDILAR, A. (2022). Antimicrobial Activities of Propolis Samples Collected From Different Provinces of Turkey. MAS Journal of Applied Sciences, 7(2), 433–442. https://doi.org/10.52520/masjaps.v7i2id195
📄Ahn, M.R., Kumazawa, S., Usui, Y., Nakamura, J., Matsuka, M., Zhu, F., Nakayama, T. 2007. Antioxidant activity and constituents of propolis collected in various areas of China, Food Chemistry, 101: 1383-1392.
📄Basim, E., Basim, H., Özcan, M. 2006. Antibacterial activities of Turkish pollen and propolis extracts against plant bacterial pathogens. J Food Eng, 77: 992–996.
📄Burdock, G.A. 1998. Review of the biological properties and toxicity of bee propolis. Food and Chemical Toxicology, 36: 347-363.
📄Bankova, V.S., De Castro, S.L., Maria, C., Marcuccı, M.C. 2000. Propolis: Recent advances in chemistry and plant origin, Apidologie, 31: 3-15.
📄Búfalo, M.C., Figueiredo, A.S., de Sousa, J.P. Candeias, J.M., Bastos, J.K., Sforcin, J.M. 2009. Anti-poliovirus activity of Baccharis dracunculifolia and propolis by cell viability determination and real-time PCR. J. Appl. Microbiol. 107(5): 1669–1680.
📄Banskota, A.H., Tezuka, Y., Adnyana, I.K., Midorikawa, K., Matsushige, K., Message, D., Huertas, A.A.G., Kadota, S. 2000. Cytotoxic, hepatoprotective and free radical scavenging effects of propolis from Brazil, Peru, the Netherlands and Chine. Journal of Etnopharmacology, 72: 239-246.
📄Barlak, Y., Değer, O., Ucar, M., Cakiroglu T.N. 2015. Effects of Turkish Propolis Extract On Secretion of Polymorphonuclear Elastase Following Respiratory Burst. Turkish Journal of Biology, 39: 194-201.
📄Bankova, V., Christov, R., Kujumgiev, A., Marcucci, M.C., Popov, S. 1995. Chemical composition and antibacterial activity of Brazilian propolis. Z Naturforsch C, 50 (3-4): 167- 72.
📄Banskota, A.H., Tezuka, Y., Adnyana, I.K., Ishii, E., Midorikawa, K., Matsushige, K., Kadota, S. 2001. Hepatoprotective and anti-Helicobacter pylori activities of constituents from Brazilian propolis. Phytomedicine, 8: 16–23.
📄Crane, E. 1997. The past and present importance of bee products to man. Bee products: properties, applications, and apitherapy. Plenum Press, New York, Pp. 1-14.
📄Chen, P.C., Wong, G. 1996. Honey bee propolis: prospects in medicine. Bee World, 77: 8–15.
📄De Groot, A.C. 2013. Propolis: a review of properties, applications, chemical composition, contact allergy, and other adverse effects. Dermatitis. 24(6): 263–282.
📄Dobrowalski, J.W., Vohora, S.B., Sharma, K., Shah, S.A., Naqvi, S.A.H., Dandiya, P.C. 1991. Antibacterial, antifungal, antiamoebic, antiinflammatory and antipyretic studies on propolis bee products. Journal of Ethnopharmacology, 35: 77-82.
📄Duran, N., Gülbol Duran, G., Öztaş, H., Özcan, B. 2010. Antımıcrobıal And Antıfungal Actıvıtıes Of Adana Propolıs. The Medical Journal of Mustafa Kemal University; 1(1): 07-14.
📄El-Guendouz, S., Al-Waili, N., Aazza, S., Elamine, Y., Zizi, S., Al-Waili, T., Lyoussi, B. 2017. Antioxidant and diuretic activity of co-administration of Capparis spinosa honey and propolis in comparison to furosemide. Asian Pac. J. Trop. Med., 10(11): 974-980.
📄Freitas, S.F., Shinohara, L., Sforcin, J.M., Guimarães, S. 2006. In vitro effects of propolis on Giardia duodenalis trophozoites. Phytomedicine, 13(3): 170-175.
📄Franchin, M. Da Cunha, M.G., Denny, C., Napimoga, M.H., Cunha, T.M., Bueno-Silva, B., Matias De Alencar, S., Ikegaki, M., Luiz Rosalen, P. 2013. Bioactive fraction of geopropolis from Melipona scutellaris decreases neutrophils migration in the inflammatory process: Involvement of nitric oxide pathway. Evid.-Based Compl. Alt. Med., 2013: 9.
📄Fernandes, Jnr. A. Sugizaki, M.F., Fogo, M.L Funari, S.R.C. Lopes, C.A.M. 1995. In vitro activity of propolis against bacterial and yeast pathogens isolated from human infections. Journal of Venomous Animals and Toxins, 1: 63-69.
📄Ghisalberti, E.L. 1978. Propolis: A Review. Bee Wld., 60: 59-84.
📄Gekker, G., Hu. S., Spivak, M., Lokensgard, J.R., Peterson, P.K. 2005. Anti-HIV-1 Activity of Propolis in CD4+ Lymphocyte and Microglial Cell Cultures, Journal of Ethnopharmacology, 102(2): 158-163.
📄Grange, J.M., Davey, R.W. 1990. Antibacterial properties of propolis (bee glue). Journal of the Royal Society of Medicine. 83(3): 159–160.
📄Hadžić, V., Jerković, A., Mujkić, A.J., Darja Husejnagi, D., Bačić, A. 2019. Antıbacterıal Actıvıty of Varıous Oıls And Ethanol Extract Propolıs Agaınst Gram Posıtıve And Gram Negatıve Bacterıa. nt. J. Adv. Res. 7(10): 141-147.
📄Hegazi, A.G., Abd El Hady, F.K., Abd Allah, F.A.M. 2000. Chemical composition and antimicrobial activity of European propolis. Zeitschrift für Naturforschung, 55: 70-75.
📄Kujumgiev, A., Tsvetkova, I., Serkedjieva, Y., Bankova, V., Christov, R., Popov, S. 1999. Antibacterial, antifungal and antiviral activity of propolis of different geographic origin. Journal of Ethnopharmacology, 64: 235-240.
📄Keskin, N., Hazir, S., Baser, K.H.C., Kürkçüoğlu, M. 2001. Antibacterial activity and chemical composition of Turkish propolis, Z. Naturforsch. C, 56: 1112–1115.
📄Kartal, M., Yıldız, S., Kaya, S., Kurucu, S., Topçu, G. 2003. Antimicrobial activity of propolis samples from two diff erent regions of Anatolia. J Ethnopharmacol, 86: 69-73.
📄İlkimen, H., Yenikaya, C., Gülbandılar, A., Sarı, M. 2016. Synthesis and characterization of a novel proton salt of 2-amino-6-nitrobenzothiazole with 2,6-pyridinedicarboxylic acid and its metal complexes and their antimicrobial and antifungal activity studies. Journal of Molecular Structure, 1120: 25-33.
📄Ilkimen, H., Gulbandilar, A. 2018. Investigation of Antimicrobial Effects of Lavender, Sage, Thyme and Chamomile Extracts, Journal of the Turkish Society of Microbiology, 48(4): 241-246.
📄Marcucci, M.C. 1995. Propolis: chemical composition, biological properties and therapeutic activity. Apidologie, 26: 83–89.
📄Marghitas, L.A. Dezmirean, D.S., Bobis, O. 2013. Important developments in Romanian propolis research. Evidence-Based Complementary and Alternative Medicine, 2013: 159392.
📄Mirzoeva, O. K., Grishanin, R. N., Calder, P.C. 1997. Antimicrobial action of propolis and some of its components: the effects on growth, membrane potential and motility of bacteria. Microbiol. Res. 152: 239–246.
📄Nieva, M.M.I., Isla, M.I., Cudmani, N.G., Vattuone, M.A., Sampietro, A.R. 1999. Screening of antibacterial activity of Amaicha del Valle (Tucuman, Argentina) propolis. J. Ethnopharmacol, 68: 97–102.
📄Park, Y.K., Koo, M.H., Abreu, J.A., Ikegaki, M., Cury, J.A., Rosalen, P.L. 1998. Antimicrobial activity of propolis on oral microorganisms. Current Microbiology, 36: 24-28.
📄Przybyłek, I., Karpiński, T.M. 2019. Antibacterial properties of propolis. Molecules. 24(11): 2047-2063.
📄Righi, A.A., Alves, T.R., Negri, G., Marques, L.M., Breyer, H., Salatino, A. 2010. Brazilian red propolis: unreported substances, antioxidant and antimicrobial activities. Journal Science Food and Agricultural, 91: 2363-2370.
📄Salatino, É., Teixeira, W., Negri, G. 2005. Origin and chemical variation of Brazilian propolis. Evidence-Based Complementary and Alternative Medicine, 2: 33-38.
📄Sforcin, J.M., Fernandes Jr, A., Lopes, C.A.M., Bankova, V., Funari, S.R.C. 2000. Seasonal effect on Brazilian propolis antibacterial activity. Journal of Ethonopharmacology, 73: 243–249.
📄Sforcin, J.M., Fernandes, Júnior, A., Lopes, C., Funari, S., Bankova, V. 2001. Seasonal effect of Brazilian propolis on Candida albicans and Candida tropicalis, Journal of Venomous Animals and Toxins, 7(1): 139-144.
📄Siheri, W., Alenezi, S., Tusiimire, J., Watson, D.G. 2017. The Chemical and Biological Properties of Propolis. Bee Products - Chemical and Biological Properties.137-178.
📄Seidel, V., Peyfoon, E., Watson, D.G., Fearnley, J. 2008. Comparative study of the antibacterial activity of propolis from different geographical and climatic zones. Phytother Res, 22: 1256- 1263.
📄Silici, S., Kutluca, S. 2005. Chemical composition and antibacterial activity of propolis collected by three different races of honeybee in the same region. Journal of Ethnopharmacology, 99: 69-73.
📄Seferoğlu, Z., Ertan, N., Yılmaz, E., Uraz, G. 2008. Synthesis, spectral characterisation and antimicrobial activity of new disazo dyes derived from heterocyclic coupling component. Coloration Technology, 124: 27-35.
📄Uzel, A., Önçağ, Ö., Çoğulu, D., Gençay, Ö. 2005. Chemical compositions and antimicrobial activities of four different Anatolian propolis samples. Microbiol Res, 160:189–195.
📄Temiz, A., Şener, A., Özkök-Tüylü, A., Sorkun, K., Salih, B. 2011. Antibacterial activity of bee propolis samples from different geographical regions of Turkey against two foodborne pathogens, Salmonella Enteritidis and Listeria monocytogenes. Turk J Biol. 35: 503-511.
📄Veiga, R.S., Mendonça, S.D., Mendes, P.B., Paulino, N., Mimica, M.J., Netto, A.A.L., Lira, I.S. López, B.G.C., Negrão, V., Marcucci, M.C. 2017. Artepillin C and phenolic compounds responsible for antimicrobial and antioxidant activity of green propolis and Baccharis dracunculifolia DC. J. Appl. Microbiol. 122: 911–920.
📄Velikova, M., Bankova, V., Sorkun, K., Houcine, S., Tsvetkova, I., Kujumgiev, A. 2000. Propolis from the Mediterranean region: chemical composition and antimicrobial activity. Z Naturforsch C, 55(9-10): 790-793.
