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Isparta Uygulamalı Bilimler Üniversitesi, Ziraat Fakültesi, Bitki Koruma Bölümü, Isparta
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Isparta Uygulamalı Bilimler Üniversitesi, Ziraat Fakültesi, Bitki Koruma Bölümü, Isparta
Abstract
This study was conducted to detect the presence of pepper mild mottle virus (PMMoV) during the 2023-2024 vegetation period in pepper cultivation areas of Isparta province. A total of 200 plant samples, suspected to be infected with PMMoV based on symptomatological observations were collected. All samples were tested using the DAS-ELISA (Double Antibody Sandwich Enzyme Linked Assay method) with antibodies specific to PMMoV. As a result of DAS-ELISA methods, 19 out of the 200 samples were found to be infected with virus. In this study, the 19 samples that tested positive by DAS-ELISA methods were used in IC-RT-PCR (Immunocapture Reverse Transcription Polimerase Chain Reaction) method. In PCR method, 836 bp fragments of RdRp gene of PMMoV was successfully amplified and a virus specific band of the expected size was observed on the agarose gel. The study confirmed the presence of PMMoV in Isparta province both and serological and molecular.
Keywords
PMMoV,pepper,detection,virus
How to Cite
AKGÜN, M. S., & ÇULAL KILIÇ, H. (2025). Detection of Pepper Mild Mottle Virus (PMMoV) in Pepper Growing Areas of Isparta Province by Serological and Molecular Methods. MAS Journal of Applied Sciences, 10(3), 428–437. https://doi.org/10.5281/zenodo.16493734
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Handan ÇULAL KILIÇ
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