The Biotechnological Potential and Treatment Processes of Cheese Whey


Abstract views: 247 / PDF downloads: 101

Authors

DOI:

https://doi.org/10.5281/zenodo.16731871

Keywords:

Cheese whey, biotechnological valorization, anaerobic digestion, membrane technologies

Abstract

The global rise in dairy product consumption has significantly increased the volume of waste generated by the dairy industry, particularly cheese whey (CW), a by-product rich in organic matter and bioactive compounds. Owing to its high chemical and biological oxygen demand, the untreated discharge of CW poses serious environmental threats. However, its rich composition, which includes proteins, lactose, and minerals, makes whey a promising raw material for environmental management as well as for the production of value-added products. This review offers a comprehensive evaluation of the biotechnological potential of whey and the treatment technologies applied since 2004. Emphasis is placed on anaerobic processes and membrane-based separation techniques, and novel integrated approaches aimed at promoting circular economy principles. The study highlights recent advances in resource recovery, biogas generation, and bioactive compound isolation, while addressing the ecological and economic implications of whey valorization through sustainable and innovative methods.

References

Antonopoulou, G., Stamatelatou, K., Venetsaneas, N., Kornaros, M., Lyberatos, G., 2008. Biohydrogen and methane production from cheese whey in a two-stage anaerobic process. Industrial & Engineering Chemistry Research, 47(15): 5227-5233.

Antonopoulou, G., Ntaikou, I., Bebelis, S., Lyberatos, G., 2021. On the evaluation of filtered and pretreated cheese whey as an electron donor in a single chamber microbial fuel cell. Biomass Conversion and Biorefinery, 11(2): 633-643.

Asunis, F., Sanna, A., Meli, V., Orsini, M., 2020. Valorization of cheese whey through biotechnological and chemical processes. Journal of Environmental Management, 275: 111231.

Blais, J.F., Drouin, M., Mercier, G., 2022. Membrane technologies for dairy industry wastewater treatment and reuse. Separation and Purification Technology, 290: 120904.

Carvalho, F., Prazeres, A.R., Rivas, J., 2013. Cheese whey wastewater: characterization and treatment. Science of the Total Environment, 446: 385–396.

Chatzipaschali, A.A., Stamatis, A.G., 2012. Biotechnological utilization of whey through fermentation using lactic acid bacteria. Biotechnology Advances, 30(5): 943–963.

Cruz-Salomón, A., Ríos-Valdovinos, E., Pola-Albores, F., Lagunas-Rivera, S., Cruz-Rodríguez, R.I., Cruz-Salomón, K.D.C., Domínguez-Espinosa, M.E., 2020. Treatment of cheese whey wastewater using an expanded granular sludge bed (EGSB) bioreactor with biomethane production. Processes, 8(8): 931.

Demirel, B., Yenigün, O., Onay, T.T., 2005. Anaerobic treatment of dairy wastewaters: a review. Process Biochemistry, 40(8): 2583–2595.

Diamantis, V.I., Kapagiannidis, A.G., Ntougias, S., Tataki, V., Melidis, P., Aivasidis, A., 2014. Two-stage CSTR–UASB digestion enables superior and alkali addition-free cheese whey treatment. Biochemical Engineering Journal, 84: 45-52.

Escalante, H., Pabón-Baquero, L., del Río, J., 2018. Cheese whey: a review of processing technologies and environmental impact. Renewable and Sustainable Energy Reviews, 93: 865–875.

Eurostat, 2024. Milk and milk product statistics. European Commission. https://ec.europa.eu/eurostat/statistics-exp lained/SEPDF/cache/29568.pdf (Accessed: 10.03.2025)

Fernández, C., Cuetos, M.J., Martínez, E.J., Gómez, X., 2015. Thermophilic anaerobic digestion of cheese whey: coupling H2 and CH4 production. Biomass and Bioenergy, 81: 55-62.

Frigon, J.C., Breton, J., Bruneau, T., Moletta, R., Guiot, S.R., 2009. The treatment of cheese whey wastewater by sequential anaerobic and aerobic steps in a single digester at pilot scale. Bioresource technology, 100(18): 4156-4163.

Gannoun, H., Bouallagui, H., Okbi, A., Sayadi, S., Hamdi, M., 2008a. Mesophilic and thermophilic anaerobic digestion of biologically pretreated cheese whey. Bioresource Technology, 99(14): 5452–5460.

Gannoun, H., Khelifi, E., Bouallagui, H., Touhami, Y., Hamdi, M., 2008b. Ecological clarification of cheese whey prior to anaerobic digestion in upflow anaerobic filter. Bioresource Technology, 99(14): 6105-6111.

Jiang, L., Wang, L., Wu, J., 2022. Improving emulsifying properties of whey protein isolate via ultrasound-assisted pH shifting. Food Hydrocolloids, 122: 107099.

Kanellos, G., Antarachas, M., Lyberatos, G., Tremouli, A., 2025. Cheese whey treatment using a microbial electrolysis cell-assisted anaerobic digestion system: the effects of pretreatment, organic loading and applied potential. Waste and Biomass Valorization, 1-16.

Kataki, S., Hazarika, S., Baruah, D.C., Kalita, P., 2016. Biogas production from anaerobic co-digestion of cow dung and food waste. International Journal of Environmental Science and Technology, 13: 1833–1840.

Kotoulas, A., Agrafioti, E., Kalderis, D., Diamadopoulos, E., 2019. Dairy wastewater treatment using constructed wetlands and advanced oxidation processes. Ecological Engineering, 138: 270–279.

Luján-Facundo, M.J., Mendoza-Roca, J.A., Cuartas-Uribe, B., 2017. Evaluation of cleaning protocols in ultrafiltration membranes fouled with whey protein solutions. Separation and Purification Technology, 172: 321–328.

Madureira, A.R., Pereira, C.I., Gomes, A.M., Pintado, M.E., Malcata, F.X., 2007. Bovine whey proteins–overview on their main biological properties. Food Research International, 40(10): 1197–1211.

Mansor, A.F., Lau, W.J., Ismail, A.F., 2021. A review on membrane technologies for treatment of dairy industry wastewater. Environmental Technology & Innovation, 22: 101443.

Mirabella, N., Castellani, V., Sala, S., 2014. Current options for the valorization of food manufacturing waste: a review. Journal of Cleaner Production, 65: 28–41.

O’Mahony, J.A., Fox, P.F., 2014. Milk proteins: introduction and historical aspects. In: Fox, P.F., McSweeney, P.L.H. (Eds.), Advanced Dairy Chemistry, Springer, pp. 1–21.

Pihlanto, A., 2011. Whey proteins and peptides. In: Mine, Y., Shahidi, F. (Eds.), Nutraceutical Proteins and Peptides in Health and Disease, CRC Press, pp. 191–201.

Pires, A., Marques, R.C., Santos, J., 2021. Valorization of cheese whey: a review on current and emerging processes. Bioresource Technology, 341: 125795.

Prazeres, A.R., Carvalho, F., Rivas, J., 2012. Cheese whey management: a review. Journal of Environmental Management, 110: 48–68.

Ramasamy, E.V., Gajalakshmi, S., Sanjeevi, R., Jithesh, M.N., Abbasi, S.A., 2004. Feasibility studies on the treatment of dairy wastewaters with upflow anaerobic sludge blanket reactors. Bioresource Technology, 93(2): 209-212.

Ramos-Suárez, J.L., Álvarez-Méndez, S.J., Tejera, E.P., Ritter, A., Gonzalez, J.M., 2024. Temperature control effect on cheese whey anaerobic digestion with low-cost tubular digesters. Processes, 12(7): 1452.

Remón, J., Ruiz, J., Oliva, M., García, L., Arauzo, J., 2016. Production of high-value products from cheese whey by thermo-catalytic conversion. Fuel Processing Technology, 144: 274–282.

Rico, C., Muñoz, N., Fernández, J., Rico, J.L., 2015. High-load anaerobic co-digestion of cheese whey and liquid fraction of dairy manure in a one-stage UASB process: Limits in co-substrates ratio and organic loading rate. Chemical Engineering Journal, 262: 794-802.

Rincón-Catalán, N.I., Pérez-Fabiel, S., Mejía-González, G., Herrera-López, D., Castro-Chan, R., Cruz-Salomón, A., Sebastian, P. J., 2022. Power generation from cheese whey treatment by anaerobic digestion and microbial fuel cell. Waste and Biomass Valorization, 13(7): 3221-3231.

Saddoud, A., Hassairi, I., Sayadi, S., 2007. Anaerobic membrane reactor with phase separation for the treatment of cheese whey. Bioresource Technology, 98(10): 2102–2108.

Smithers, G.W., 2008. Whey and whey proteins from‘gutter-to-gold’. International Dairy Journal, 18(7): 695–704.

Smithers, G.W., 2015. Whey-ing up the options–Yesterday, today and tomorrow. International Dairy Journal, 48: 2-14.

Talebi, S., Ranjbar, M., Dehghani, M., 2020. Membrane technologies for dairy wastewater treatment and reuse: a review. Journal of Environmental Chemical Engineering, 8(6): 104514.

Tirado, D.F., Hernández, A.M., Gómez, C.A., 2018. Electrocoagulation for the treatment of dairy wastewater: performance and cost evaluation. Environmental Science and Pollution Research, 25: 31677–31687.

Valta, K., Kosanovic, T., Malamis, D., Moustakas, K., Loizidou, M., 2017. Overview of cheese whey management and recovery options. Journal of Cleaner Production, 140: 753–762.

Valta, K., Kosanovic, T., Malamis, D., Moustakas, K., Loizidou, M., 2015. Overview of water usage and wastewater management in the food and beverage industry. Desalination and Water Treatment, 53(12): 3335-3347.

Venetsaneas, N., Antonopoulou, G., Stamatelatou, K., Kornaros, M., Lyberatos, G., 2009. Using cheese whey for hydrogen and methane generation in a two-stage continuous process with alternative pH controlling approaches. Bioresource technology, 100(15): 3713-3717.

Yetilmezsoy, K., Ilhan, F., Zengin, Z., 2017. Recovery of nutrients from anaerobic digestate of poultry manure via struvite precipitation: optimization, evaluation and kinetic modeling. Bioresource Technology, 239: 403–413.

Yorgun, M.S., Balcioglu, I.A., Saygin, O., 2008. Performance comparison of ultrafiltration, nanofiltration and reverse osmosis on whey treatment. Desalination, 229(1-3): 204-216.

Downloads

Published

2025-09-10

How to Cite

ÇELİKTEN, H. (2025). The Biotechnological Potential and Treatment Processes of Cheese Whey. MAS Journal of Applied Sciences, 10(3), 446–455. https://doi.org/10.5281/zenodo.16731871

Issue

Section

Articles