Genetic Diversity of Genotypes that can be used as Genitors in Bean Breeding Programs
Abstract views: 21
/ 
PDF downloads: 18
DOI:
https://doi.org/10.5281/zenodo.15620345Keywords:
Bean genotypes, molecular characterization, bean breeding, SRAP markersAbstract
The study was carried out at Ege University Faculty of Agriculture, Department of Horticulture, Research and Application Garden, molecular characterization of bean genotypes for which morphological and agronomic characterization was completed was carried out and their usability in bean breeding programs was examined. For this purpose, a total of 55 common bean genotypes grown in different regions of Turkey, especially in the Aegean and Marmara Regions, and obtained from other countries of the world were examined. After the genotypes were evaluated in terms of morphological and agronomic characteristics including plant, flower, pod and seed, molecular characterization was carried out using SRAP markers to reveal the genetic variation of the genotypes. As a result of the analysis using 32 SRAP marker combinations, 83.03% of the variation was included in 4 factor groups and the genetic distance of the genotypes ranged between 61% and 98%.
References
Alghamdi, S.S., Al-Faifi, S.A., Migdadi, H.M., Khan, M.A., El-Harty, E.H., Ammar, M.H., 2012. Molecular diversity assessment using sequence related amplified polymorphism (SRAP) markers in Vicia faba L. International Journal of Molecular Sciences, 13(12): 16457-16471.
Amanullah, K.A., Nawab, K., Sohail, Q., 2006. Performance of promising common bean (Phaseolus vulgaris L.) germplasm at Kalam-Swat. Pakistan Journal of Biological Sciences, 9(14): 2642–2646.
Anushri, V., Mohapatra, T., Sharma, R.P., 2004. Plant Biotechnology and molecular markers. (Ed: P.S. Srivatava, Alka Narula and Sheela Srivastava). Anamaya Publishers, New Delhi, India.
Baloch, F.S., Kurt, C., Arıoğlu, H., Özkan, H., 2010. Assaying of diversity among soybean (Glycin max (L.) Merr.) and peanut (Arachis hypogaea L.) genotypes at DNA level. Turkish Journal of Agriculture and Forestry, 34: 285-301.
Becerra-Velásquez, V.L., Gepts, P., 1994. RFLP diversity in common bean (Phaseolus vulgaris L.). Genome, 37: 256-263.
Broughton, W.J., Hernandez, G., Blair, M., Beebe, S., Gepts, P., Vanderleyden, J., 2003. Beans (Phaseolus spp.) - model food legumes. Plant and Soil, 252: 55-128.
Burle, M.L., Fonseca, J.R., Kami, J.A., Gepts, P., 2010. Micro-Satellite diversity and genetic structure among common bean (Phaseolus vulgaris L.) landraces in Brazil, a secondary center of diversity. Theoretical and Applied Genetics, 121:801-813.
Castro-Guerrero, N.A., Isidra-Arellano, M.C., Mendoza-Cozatl, D.G., Valdés-López, O., 2016. Common bean: a legume model on the rise for unraveling responses and adaptations to iron, zinc and phosphate deficiencies. Frontiers in Plant Science, 7: 600.
Catarcione, G., Paolacci, A.R., Alicandri, E., Gramiccia, E., Taviani, P., Rea, R., Costanza, M.T., De Lorenzis, G., Puccio, G., Mercati, F., Ciaffi, M., 2023. Genetic diversity and population structure of common bean (Phaseolus vulgaris L.) landraces in the Lazio Region of Italy. Plants, 12: 744.
Certel, B.T., İkten H., Yilmaz, Y., Kantar, F., Çiftçi, V., Gözen, V., Tepe, A., 2023. Molecular characterization of cold tolerant germplasm of Phaseolus beans with sequence related amplified polymorphism (SRAP) and retrotransposon-based interprımer binding sıtes (IPBS) markers. Journal of Animal and Plant Sciences, 33(3): 620-632.
Ceylan, A., Öcal, N., Akbulut, M., 2014. Genetic diversity among the Turkish common bean cultivars (Phaseolus vulgaris L.) as assessed by SRAP, POGP and cpSSR markers. Biochemical. Systematics and Ecology, 54: 219-229.
Chandrakanth, E., Hall T.C., 2008. Phaseolin: structure and evolution. The Open Evolution Journal, 2: 66-74.
Garcia, R.A.V., Rangel, P.N., Brondani, C., Martins, W.S., Melo, L.C., Carneiro, M.S., Borba, T.C.O., Brondani, R.P.V., 2011. The characterization of a new set of est-derived simple sequence repeat (SSR) markers as a resource for the genetic analysis of Phaseolus vulgaris. BMC Genetics,12(41): 1471-2156.
Gepts, P., Debouck, D.G., 1991. Origin, domestication and evolution of the common bean, Phaseolus vulgaris. In: (Ed: O Voyset and A. Van Schoonhoven). Common Beans: Research for Crop Improvement. CAB International, Wallingford, Oxon, pp. 7-53.
Gepts, P., Arag˜ao, F., Barros, E., Blair, M.W., Brondani, R., Broughton, W., Galasso, I., Hern´andez, G., Kami, J., Lariguet, P., McClean, P., Melotto, M., Miklas, P., Pauls, P., Pedrosa-Harand, A., Porch, T., S´anchez, F., Sparvoli, F., Yu, K., 2008. Genomics of Phaseolus beans, a major source of dietary protein and micronutrients in the tropics. (Ed: Moore, P., Ming, R.), Genomics of Tropical Crop Plants, Springer, pp. 113-143.
Geravandi, M., Cheghamirza, K., Farshadfar, E., Gepts, P., 2020. QTL analysis of seed size and yield-related traits in an inter-genepool population of common bean (Phaseolus vulgaris L.). Scientia Horticulturae, 274: 109678.
Guindon, M.F., Martin, E., Zayas, A., Cointry, E., Cravero, V., 2016. Evaluation of SRAP markers for mapping of Pisum sativum L. Crop Breeding and Applied Biotechnology, 16:182-188.
Guo, Y.Z., Huang, Q., Ye, X., Chen, Z., Huang, Z., Chen, H., Yan, D., Ahuzheng, X., 2023. Morphological and SRAP markers-based genetic diversity determination on Phaseolus lunatus L. Germplasms (J). Fujian Journal of Agricultural Sciences，38(2):166-173.
Esposito, M.A., Martin, E.A., Craverom, V.P., Cointry, E., 2007. Characterization of pea accessions by SRAP’s markers. Scientia Horticulturae, 113: 329-335.
Mohammed, N.A., Afzal, M., Al-Faifi, S.A., Khan, M.A., Refay, Y.A., Al-Samin, B.H., Ibrahim, A., 2023. Effectiveness of Sequence-Related Amplified Polymorphism (SRAP) markers to assess the geographical origin and genetic diversity of collected lentil genotypes. Plant Biotechnology Reports, 17: 519-530.
Jannat, S., Shah, A.H., Ul Hassan, M., Sher, A., Fiaz, S., Elesawy, B.H., Ismail, K.A., El Askary, A., Gharib, A.F., Qayyum, A., 2022. Genetic diversity of common bean (Phaseolus vulgaris L.) ecotypes from Pakistan using Simple Sequence Repeats, Saudi Journal of Biological Sciences, 29.
Katungi, E., Sperling, L., Karanja, D., Farrow, A., Beebe, S., 2011. Relative importance of common bean attributes and variety demand in the drought areas of Kenya. Journal of Development Agricultural Economics, 3 (8): 411-422.
Kwak, M., Gepts, P., 2009. Structure of genetic diversity in the two major gene pools of common bean (Phaseolus vulgaris L. Fabaceae). Theoretical Applied Genetics, 118: 979-992.
Li, G., Quiros, C.F., 2001. Sequence-related amplified polymorphism (SRAP), a new marker system based on a simple pcr reaction: its application to mapping and gene tagging in Brassica. Theoretical Applied Genetics, 103: 455-461.
Madakbaş, S.Y., Ergin, M., 2011. Morphological and phenological characterization of Turkish bean (Phaseolus vulgaris L.) genotypes and their present variation states. African Journal of Agricultural Research, 6(28): 6155-6166.
Martins, S.R., Vences, F.J., Miera, E.S., Barroso, M.R., Carnide, V., 2006. RAPD analysis of genetic diversity among and within Portuguese landraces of common white bean (Phaseolus vulgaris L.). Scientia Horticulturae, 108(2): 133-142.
Pallottini, L., Garcia, E., Kami, J., Barcaccia, G., Gepts, P., 2004. The genetic anatomy of a patented yellow bean. Crop Science, 44: 968-977.
Rheenen, H.A., 1979. Diversity of food beans in Kenya. Economic Botany, 33(4): 448-454.
Mercati, F., Leone, M., Lupini, A. Sorgonà, A., Bacchi M., Abenavoli M.R., Sunseri F., 2013. Genetic diversity and population structure of a common bean (Phaseolus vulgaris L.) collection from Calabria (Italy). Genet Resources and Crop Evolution, 60: 839-852.
Miklas, P.N., Stone, V., Daly, M.J., Stavely, J.R., Steadman, J.R., Bassett, M.J., Delorme R., Beaver, J.S., 2000. Bacterial, fungal and viral disease resistance loci mapped in a recombinant inbred common bean population ('Dorado'/XAN 176). Journal of the American Society for Horticultural Science, 125: 476-481.
Miklas, P.N., Hu, J., Niklaus, G.J., Larsen, K.M., 2006. Potential application of TRAP (Targeted Region Amplified Polymorphism) markers for mapping and tagging disease resistance traits in common bean. Genomics, Molecular Genetics and Biotechnology, 46(2): 910-916.
Mukeshimana, G., Pañeda, A., Rodriguez, C., Ferreira, J.J., Giraldez, R., Kelly, J.D., 2005. Markers linked to the bc-3 gene conditioning resistance to bean common mosaic potyviruses in common bean. Euphytica, 144: 291-299.
Naderpour, M., Lund, O.S., Larsen, R., Johansen E., 2010. Potyviral resistance derived from cultivars of Phaseolus vulgaris carrying bc-3 is associated with the homozygotic presence of a mutated eIF4E allele. Molecular Plant Pathology, 11: 255-263.
Sustar-Vozlic, J., Maras, M., Javornik, B., Meglic, V., 2006. Genetic diversity and origin of Slovene common bean (Phaseolus vulgaris L.) germplasm as revealed by AFLP markers and phaseolin analysis. Journal of the American Society for Horticultural Science, 131(2): 242-249.
Saghai-Maroof, M.A., Soliman, K.M., Jorgensen, R.A., Allard, R.W., 1984. Ribosomal DNA spacer-length polymorphisms in barley: Mendelian inheritance, chromosomal location and population dynamics. The Proceedings of the National Academy of Sciences, USA, 81(24): 8014-8018.
Tiwari, M., Singh, N.K., Rathore, M., Kumar, N., 2005. RAPD markers in the analysis of genetic diversity among common bean germplasm from central Himalaya. Genetic Resources and Crop Evolution, 52(3): 315-324.
Villa, T., Maxted, N., Scholten, M., Ford-Lloyd, B., 2006. Defining and identifying crop landraces. Plant Genetic Resources, 3(3): 373-384.
Zeven, A.C., Waninge, J., Van Hintum, T.H., Singh, S.P., 1999. Phenotypic variation in a core collection of common bean (Phaseolus vulgaris L.) in the Netherlands. Euphytica, 109: 93-106.
Downloads
Published
How to Cite
Issue
Section
License
Copyright (c) 2025 The copyright of the published article belongs to its author.
This work is licensed under a Creative Commons Attribution-NonCommercial 4.0 International License.
The journal is licensed under a 